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白细胞介素-10对人牙囊细胞OPG表达的影响 被引量：4

Regulation of OPG secretion by IL-10 in human dental follicle cells.
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摘要 目的:研究人牙囊细胞白细胞介素-10(IL-10)蛋白的表达及其对骨保护素(osteoprotegerin,OPG)表达的影响。方法:采用组织块加胶原酶消化法培养人牙囊细胞,进行IL-10免疫组化染色。将25ng/mlIL-10作用于牙囊细胞0h、1h、3h、6h、9h,用ELISA法检测上清液中OPG含量变化。结果:人牙囊细胞IL-10表达阳性。25ng/mlIL-10作用于人牙囊细胞3～6hOPG表达显著增加(p<0.05)。结论:人牙囊细胞表达IL-10,IL-10通过增加人牙囊细胞OPG表达,在牙齿萌出过程中起重要的调控作用。 Objective：To study the expression of IL-10 at protein level in human dental follicle cells （HDFCs）and the effect of IL-10 on osteoprotegerin （OPG） secretion. Method： HDFCs were cultured by explant method. The expression of IL-10 at protein level was tested by immunocytochemistry and OPG secretion treated with 25 ng/ml IL-10 at 0, 1,3,6,9 h was examined by ELISA method. Result： IL-10 was expressed in the cytoplasm of HDFCs. OPG secretion was up-regulated by IL-10 at 3-6h（p〈0.05）. Conclusion：IL-10 may regulate tooth eruption by up-regulating OPG secretion.
作者 钱红 段银钟 金作林 陈学鹏 毕迎春
机构地区 第四军医大学口腔医院正畸科
出处 《临床口腔医学杂志》 2007年第2期69-71,共3页 Journal of Clinical Stomatology
基金 国家自然科学基金资助项目(30400510)
关键词 人牙囊细胞 白细胞介素-10 骨保护素 牙齿萌出 human dental follicle cells interleukin-10（IL-10） osteoprotegerin（OPG） tooth eruption
分类号 R575.2 [医药卫生—消化系统]
  • 相关文献

参考文献13

  • 1Wise GE,Frazier-Bowers S,DSouza RN.Cellular,molecular,and genetic determinants of tooth eruptio[J].Crit Rev Oral Biol Med,2002,13 (4):323-334.
  • 2孙海燕,林珠,金作林,张永宽.CSF-1和PTHrP对人牙囊细胞OPG表达的影响[J].临床口腔医学杂志,2005,21(12):709-712. 被引量：4
  • 3孙海燕,林珠,金作林,张永宽.巨噬细胞集落刺激因子对体外培养人牙囊细胞破骨细胞分化因子表达的影响[J].牙体牙髓牙周病学杂志,2006,16(4):183-187. 被引量：5
  • 4Al-Rasheed A,Scheerens H,Rennick DM,et al.Accelerated alveolar bone loss in mice lacking interleukin-10[J].J Dent Res,2003,82(8):632-635.
  • 5Deschner J,Arnold B,Kage A,et al.Suppression of interleukin-10 release from human periodontal ligament cells by interleukin-1β in vitro[J].Arch Oral Biol,2000,45(2):179-183.
  • 6Liu D,Yao S,Wise GE.Effect of interleukin-10 on gene expression of osteoclastogenic regulatory molecules in the rat dental follicle[J].Eur J Oral Sci,2006,114(1):42-49.
  • 7Suzuki T,Suda N.Ohyama.Osteoclastogenesis during mouse tooth germ development is mediated by receptor activator of NFk-B ligand(RANKL)[J].J Bone Miner Metab,2004,22 (3):185-191.
  • 8Mizuno A,Amizuka N,Irie K,et al.Severe osteoporosis in mice lacking osteoclastogenesis inhibitory factor/ osteoprotegerin[J].Biochem Biophys Res Commun,1998,247 (3):610-615.
  • 9Wise GE,Lumpkin SJ,Huang H,et al.Osteoprotegerin and osteoclast differentiation factor in tooth eruption[J].J Dent Res,2000,79(12):1937-42.
  • 10Wise GE,Yao S,Liu D.Injections of Osteoprotegerin and PMA delay tooth eruption[J].Clin Anat,2006,19(1):19-24.

二级参考文献21

  • 1Cahill DR, Marks SC Jr. Tooth eruption, Evidence for the central role of the dental follicle[ J ]. J Oral Pathol, 1980,9 (4):189- 200.
  • 2Wise GE, Lin F. The molecular biology of initiation of tooth eruption[ J ]. J Dent Res, 1995,74 ( 1 ) : 303 - 306.
  • 3Wise G. E, Lin F. Regulation and localization of colony-stimulating factor-1 mRNA in cultured rat dental follicle cells[J]. Arehs Oral Biol, 1994,39(7) :621 - 627.
  • 4H Kanzaki, M Chiba, Y Shimizu, et al. Dual regulation of osteoelast differentiation by periodontal ligament eells through RANKL stimulation and OPG inhibition [ J ]. Journal of Dental Researeh, 2001,80(3) : 887.
  • 5Philbriek WM, Wysolmerski J J, Galbraith S, et al. Defining the roles of parathyroid hormone-related protein in normal physiology [J].Physiol Rev, 1996,76 ( 1 ) : 127 - 173.
  • 6Fiaschi-Taesch NM, Stewart AF. Minireview: Parathyroid hormonerelated protein as an intracrine factor-trafficking mechanisms and functional consequences [ J ]. Endocrinology, 2003, 144 ( 2 ) : 407 -411.
  • 7Mekaapiruk K, Suda N, Hammond VE, et al. The influence of parathyroid hormone-related protein PTHrP on tooth germ development and osteoclastogenesis in alveolar bone of PTHrP-knoek out and wild-type mice in vitro[J]. Arch Oral Biol,2002,47(9) :665 - 672.
  • 8Wise GE, Ding D, Yao S. Regulation of secretion of osteoprotegerin in ratdental folliele eells[J]. Eur J Oral Sci,2004,112(5) :439 - 444.
  • 9Wada N, Maeda H, Tanabe K, et al. Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function :the factor is osteoprotegerin/osteoclastogenesis inhibitory factor[J]. J Periodontal Res, 2001,36 ( 1 ) : 56 - 63.
  • 10Wise GE, Lumpkin SJ, Huang H,et al. Osteoprotegerin and osteoclast differentiation factor in tooth eruption [ J ]. J Dent Res, 2000,79(12) :1937 - 1942.

共引文献5

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二级引证文献6

临床口腔医学杂志
2007年 第2期

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