摘要
目的探讨人类错配修复基因hMSH2及增殖细胞核抗原PCNA在肺癌组织中的表达及意义。方法运用免疫组织化学S-P法对56例肺癌组织中的Hmsh2及PCNA的表达进行检测。结果56例肺癌组织中Hmsh2阳性表达率为28.6%,分化程度高者阳性率显著高于分化程度低者(P<0.01),有淋巴结转移者Hmsh2阳性率低于无淋巴结转移者(P<0.05),不同病理组织学类型之间Hmsh2表达无显著差别(P>0.05);56例肺癌组织中分化程度高者PCNA标记指数高于分化程度低者(P<0.01),有淋巴结转移者PCNA标记指数高于无淋巴结转移者(P<0.05),hMSH2阴性表达者的PCNA标记指数明显高于hMSH2阳性表达者(P<0.01),不同病理组织学类型之间PCNA标记指数无显著差别(P>0.05)。结论hMSH2基因的缺陷及PCNA的表达与肺癌的发生发展过程并与分化程度及有否淋巴结转移有关。
To investigate the expression and significance of human mismatch repair gene (hMSH2) and proliferating cell nuclear antigen (PCNA) in lung cancer. Expression of hMSH2 and PCNA in 56 cases with lung cancer was examined by S-P immunohistochemical staining. In 56 cases with lung cancer, the positive expression rate of hMSH2 was 28.6%. The positive rate of Hmsh2 Expression in the patients with high and middle differention grades was significantly higher than that of the others (P<0.01), meanwihile, the positive rate of cases with lymph node metastasis (P<0.05). It wasn′t positively correlated with pathological types. In 56 cases with lung cancer, PCNA labeling index (PCNA-LI) in the patients with high and middle differention grades was lower than that of the others (P<0.01), meanwhile, the PCNA-LI of patients with lymph node metastasis was significantly higher than that of patients without metastasis (P<0.05). PCAN-LI in hMSH2 positive lung cancer tissue was higher than that in hMSH2 negative tissue (P<0.01). [Conclusions] The abnormal expression of hMSH2 and PCNA may be involved in the carcinogens process of lung cancer and correlated with differentiation grades and lymph node metastasis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第5期691-694,共4页
China Journal of Modern Medicine
