摘要
目的探讨体外分离培养结膜上皮细胞的方法。方法用组织块培养法将人眼结膜上皮组织分别种植于培养皿及处理过的羊膜上,培养2周。观察结膜上皮细胞的生长特点。结果接种在培养皿的结膜上皮6天左右组织块之间可融合成膜状,细胞为单层上皮细胞。接种在羊膜上的结膜组织,生长较慢,2周左右组织块之间可互相连接,融合成膜状,细胞为复层上皮细胞。免疫组化鉴定结膜上皮细胞CK13染色阳性。结论组织块培养法是结膜上皮细胞培养的较好方法,种植在羊膜上可获得复层上皮细胞。
OBJECTIVE To study the culture method of conjunctival epithelium cells in vitro.METHODS The human conjunctival epithelium cells were cultured by tissue inoculation and were explanted in culture dish or on human amniotic membrane. The cell morphology and growth features were observed. RESULTS The human conjunctival epithelium cells explanted in culture dish connected each other into membrane in 1 week, and only monolayer cells were observed.The human conjunctival epithelium cells explanted on human amniotic membrane connected each other into membrane in 2 weeks, and multi - layer cells were observed. All cultured cells showed positive in CK13 staining by indirect immunofluorescence staining. CONCLUSIONS Tissue inoculation is a good approach of conjunctival epithelium cell culture, and multi - layer cells can be obtained if the tissue is explanted on human amniotic membrane.
出处
《中国中医眼科杂志》
2007年第2期81-83,共3页
China Journal of Chinese Ophthalmology
关键词
细胞培养
结膜上皮
干细胞
免疫组化
cell culture
conjunctival epithelium
stem cell
immunohistochemistry
