摘要
本文研究了双氢青蒿素的β异构体与α异构体转化平衡条件；并采用反相高效液相色谱法对其进行定量测定。色谱条件为：ＹＷＧ－Ｃ１８色谱柱（２００ｍｍ×５ｍｍ，１０μｍ），乙腈－０．０２ｍｏ１／Ｌ硫酸铵溶液－１２％三乙胺溶液（６０：４０：０．２，ｐＨ５．０）为流动相，以萘作内标，检测波长为２１０ｎｍ，本法准确、简便、快速。
This paper described the study of the conditions of conversion in equilibrium between β-dihydroartemisinin and α-dihydroartemisinin, and the determination of dihydroartemisinin by reversed phase HPLC. Naphthalene was used as internal standard and YWG─C_18 (10μm, 20cm×5mm) as column. The mobile phase consisted of a mixture of acetonitrile 0. 02mol/L ammonium sulfatel2/00 triethylamine (60 : 40 : 0. 2, PHS. 0) . The wavelength of detection was 210nm. This method is accurate, simple and rapid.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1996年第3期172-175,共4页
Chinese Journal of Pharmaceutical Analysis
