摘要
从卵巢癌腹水和肺癌胸水中分离出肿瘤浸润淋巴细胞（ＴＩＬ），用ｒＩＬ－２诱导培养，观察其增殖活性。同时观察了ＰＪ－ＣＷ、ＰＯＧＰ、ＰＨＡ等对其杀伤活性的调节作用。结果表明，在培养５～１０天时ＴＩＬ增殖较快，其表面产生的ＣＤ３、ＣＤ４、ＣＤ８、Ｔａｃ等抗原，随培养时间的延长，表达量增加，尤以ＣＤ８增加较明显，ＣＤ４／ＣＤ８比值逐降。其表面产生的ＨＬＡ－ＤＲ、ＨＬＡ－ＡＢＣ抗原，在培养１５天内有增加表现，以后下降。其杀伤活性在培养５～１０天较高，其后活性下降。在培养系统中加入ＰＪ－ＣＷ、ＰＯＧＰ、ＰＨＡ等后，培养５天时，ＴＩＬ杀伤活性分别为８２．７０％，８５．５０％，８４．３５％（对照组为内保持较高的杀伤活性。
Tumor infiltration lymphocytes (TIL) were separated from ovary cancer ascites and lung cancer pleural effusion , and cultured with rlL -2. We observed the dynamics of its proliferation activity , production of surface antigens and killing activity ,in addition ,the regulation effect of PJ-CW ,POGP ,PHA on the killing activity of TIL we were also observed. It showed iT IL proliferated fast at culture for 5-10 days , with prolongation of the culture time ,production of CD3 , 4 , 8 ,Tac antigen ,especially CD8 increased ,so the ratio of CD4 /CD8 decreased gradually. The production of HLA -DR , HLA-ABC antigens increased within culture for 15 days , and then decreased . The killing activity increased and reached the top at culture for 5 days ,and then decreased. Adding PJ-CW ,POGP and PHA into the culture medium , the killing activity of TIL at culture for 5-10 days was 82.70% , 85.50% and 84. 35% respectively ,higher than that of control (66. 80%) obviously, and maintained higher killing activity within 20 days.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1996年第3期168-170,共3页
Chinese Journal of Microbiology and Immunology
关键词
肿瘤浸润
淋巴细胞
胸腹水
肿瘤
生物活性
Tumor infiltration cymphocytes (TIL)
PJ-CW
POGP
PHA
Killing activity
