摘要
目的：检测妊娠不同时期抑制素、激活素和卵泡抑素系统在胎盘中的定位及表达水平的变化，探讨其与妊娠进展的关系。方法：选取妊娠早期（6—12周）、中期（13—28周）、晚期（37—40周）无合并症和并发症孕妇各30例为研究对象。留取胎盘绒毛组织，应用免疫组织化学技术和计算机图像分析系统检测抑制素A、抑制素B、激活素A、激活素B、卵泡抑素在妊娠不同时期胎盘绒毛组织中的表达。结果：抑制素、激活素和卵泡抑素在妊娠早期、中期、晚期胎盘绒毛的合体滋养细胞、细胞滋养细胞、毛细血管内皮细胞的胞浆呈不同程度的阳性染色。定量分析显示，随着妊娠的进展，胎盘绒毛组织中抑制素A、激活素A表达水平逐渐升高，差异有统计学意义（P〈0．05），且与妊娠时间成正相关关系（r=0．97，r=0．80；P〈0，01）；而抑制素B、激活素B和卵泡抑素的表达在妊娠早、中、晚期差异无统计学意义（P〉0．05），激活素B与妊娠时间无相关关系（r=-0．31，P〉0．05）、抑制素B和卵泡抑素表达与妊娠时间呈负相关（r=-0．47，r=-0．38；P〈0．05）。结论：抑制素、激活素和卵泡抑素系统的动态平衡对于正常妊娠的维持起重要作用。
Objective ： To study the position and expression of inhibin - activin - follistatin system at the villus of the normal first, the second and the third trimester pregnancy, and the effect of inhibin - activin - follistatin on sustaining the normal pregnancy development. Methods ： 90 pregnancy women were collected, including 30 cases of the first trimester pregnancy, 30 cases of the second trimester pregnancy and 30 cases of third trimester pregnancy without pregnancy complications. Immunohistochem/stry technology and computer image analysis systems were used to detect the expression of inhibin - activin - follistatin system in the villus. Results ： Inhibin, activin and follistatin exhibited positive staining at the syncytiotrophoblast, cytotrophoblast and capillary endothelial cell of villus. Going along with pregnancy progress, the quantitative analysis showed that the expression levels of inhibin A and activin A in the villus not only gradually increased which appeared significantly different （ P〈0.05 ）, but also positively correlated with pregnancy weeks （ r = 0.97, r =0.80 ; P 〈0.01 ）. There were no significant differences between the expression of inhibin B, activin B and follistatin in the villus with the normal first, the second and the third trimester pregnancy（ P 〉 0.05 ）. No correlation was seen between the expression of activin B in the villus with pregnancy weeks （ r = - 0.31, P〉0.05 ）. A negative correlation was seen between the expression of inhibin B and follistatin in the villus with pregnancy weeks （ r = - 0.47, r = -0. 38 ; P 〈0. 05 ）. Conclusion ： The dynamic balance of inhibin - activin - folhstatin system in placenta tissue can play important roles for sustaining the normal pregnancy.
出处
《中国计划生育学杂志》
北大核心
2007年第3期161-164,共4页
Chinese Journal of Family Planning
