摘要
目的探讨龙血素B对大鼠背根神经节细胞辣椒素诱发的辣椒素受体电流的影响。方法采用全细胞膜片钳技术在急性分离的大鼠背根神经节细胞上观察龙血素B对辣椒素诱发的辣椒素受体电流的影响。结果①在-60mV的钳制电位下,辣椒素受体拮抗剂辣椒卓平可以完全抑制辣椒素受体电流;②不同浓度的龙血素B溶液对辣椒素诱发的受体电流具有浓度依赖的抑制作用;2.0、4.0、8.0和16.0μmol.L-1的龙血素B溶液对辣椒素诱发的受体电流峰值的抑制率分别为15.36%±2.12%、36.41%±2.43%、76.26%±2.16%和96.69%±3.21%(n=10,P<0.05),半数抑制浓度(IC50)为4.9μmol.L-1,Hill系数为2.29。结论龙血素B可以明显抑制辣椒素诱发的辣椒素受体电流,影响痛觉信息的传入,这可能是以龙血素B为重要成分的中药血竭产生镇痛作用的机制之一。
Aim To investigate effects of loureirin B on capsaicin-evoked currents in rat dorsal root ganglion （DRG） neurons. Methods In acutely isolated rat DRG neurons, effects of loureirin B on capsaicin-evoked currents were observed using whole-cell patchclamp technique. Results （1） The holding potential was maintained at -60 mV and VR1 antagonist capsazepine inhibited capsaicin-evoked currents completely; （2） Loureirin B concentration-dependently inhibited capsaicin-evoked currents. Loureirin B at the concentrations of 2.0, 4.0, 8.0 and 16. 0 μmol ·L^-1 reduced capsaicin-evoked currents by 15.36% ±2. 12% ,36.41% ±2.43%,76.26% ± 2. 16% and 96.69% ±3.21% （n=10, P〈0.05） of the control respectively with haft-maximal inhibitory concentration （ IC50 ） of 4. 9 μmol ·L^-1, Hill coefficient of 2.29. Conclusion Loureirin B could markedly inhibit eapsaiein-evoked currents and block the transmission of pain sensation, which may contribute to one of analgesic mechanisms of dragon＇s blood （ its important component is loureirin B）.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2007年第2期211-214,共4页
Chinese Pharmacological Bulletin
基金
国家民委基金重点资助项目(NoMZY06002)
关键词
龙血素B
辣椒素
全细胞膜片钳技术
背根神经节
loureirin B
capsaicin
whole-cell patchclamp technique
dorsal root ganglion neuron
