摘要
目的:探讨Graves眼病患者与细胞毒性T淋巴细胞相关抗原-4(CTLA-4)基因第1外显子A49G多态性及临床特点的关系。方法:应用聚合酶链反应-限制性片段长度多态性技术测定107例Graves病患者(其中伴突眼50例,不伴眼病57例)CTLA-4基因第1外显子A49G多态性,分析其基因表型、基因频率与眼病的关系;并分析眼病与年龄、性别、甲状腺肿大程度、血清甲状腺球蛋白抗体(TgAb)、甲状腺过氧化物酶抗体(TPOAb)等临床相关因素的关系。结果:无眼病组(GH组)与伴突眼组(GO组)相比,GG基因型及等位基因G的频率差异均有显著性(P<0.05),低TgAb可能是Graves病患者伴发突眼的危险因素(P<0.05),而平均年龄、性别比例、甲状腺肿大程度以及血清TPOAb水平相比较则均无统计学差异(P>0.05)。结论:GG基因型和等位基因G是Graves眼病的遗传易感因素,低TgAb促进Graves眼病的发生;而Graves眼病的发病与年龄、性别、甲状腺肿大程度以及血TPOAb水平可能无明显相关性。
Objective To explore whether CTLA-4 gene A/G polymorphism at position 49 in exon 1 and its clinical characteristics related to Graves ophthalmopathy （GO）. Methods One hundred and seven Graves disease（GD） patients, including 50 cases with GO and 57 without GO were studied, CTLA-4 gene A/G polymorphism at position 49 in exon 1 was detected with polymerase chain reaction--restriction fragment length polymorphism （PCR-RFLP） analysis. The genotype, allele frequency and its relationship with GO were analyzed. At the same time, the relationships of age,sex, the severity of goiter, TgAb and TPOAb were also compared with GO. Results GG genotype and G allele frequency in Graves ophthalmopathy group （GO group） were significantly higher than those in Graves hyperthyroidism without ophthalmopathy group （GH group） （P 〈 0.05）; there were no differences in mean age, sex proportion, the severity of goiter, the level of TPOAb between them （P 〉 0.05） ; but the lower level of TgAb was a risk factor for GO. Conclusions GG genotype and G allele confer genetic susceptibility to GO. The lower level of TgAb contributes to GO; but the non-genetic factors, such as age, sex, the severity of goiter, the level of TPOAb,are not associated with the development of GO.
出处
《实用医学杂志》
CAS
2007年第5期642-644,共3页
The Journal of Practical Medicine
