摘要
目的:探讨术前鞘内注射选择性环氧合酶-1(cyclooxygenase-1)抑制剂SC-560对术后疼痛大鼠机械痛觉超敏以及脊髓中磷酸化ERK(p-ERK)蛋白表达的影响。方法:采用术后疼痛模型,于术后1 h通过观察机械缩足反射阈值(MWT)测定术后痛觉超敏情况、免疫组化染色和免疫印迹检测脊髓中p-ERK表达的变化。结果:①术后疼痛大鼠术后1 h出现明显痛觉超敏反应,术前鞘内注射SC-560 100μg可以明显抑制术后痛觉超敏;②术后1 h大鼠腰段脊髓背角浅层p-ERK免疫组化染色阳性细胞数较正常对照组明显增加,鞘内注射SC-560可明显减少p-ERK阳性细胞数(P<0.01);术后1 h大鼠腰段脊髓Western blot检测,p-ERK1/2蛋白的表达较正常大鼠均明显增加,鞘内注射SC-560可以抑制p-ERK1/2蛋白的表达。结论:鞘内注射COX-1抑制剂可以抑制术后疼痛大鼠术后痛觉超敏反应,脊髓水平ERK可能介导其上述作用。
Aim： To explore the effects of intrathecal injection of selective cyclooxygenase-1 （COX-1） inhibitor, SC-560, on mechanical allodynla and spinal ERK protein expression in rats with postoperative pain. Methods： Rats were divided into 4 groups： control group, postoperative pain group, SC-560 group and DMSO group. Mechanical withdrawal threshold （MWT）, immunohistoehernical and Western blotting technique were used to evaluate mechanical hypersensitivity and the expression of phospho-ERK in the spinal cord, respectively. Results： ①Behavior test rats developed allodynia 1 h after operation and SC-560 100 μg administrated intrathecally demonstrated a significant reduction in postope rative hypersensitivity. ②Immunohistoehernical staining Phospho-ERK positive neurons in the rat superficial spinal dorsal horn increased significantly 1 h after incision compared with that of non-incision group. Intratheeal administration of SC-560 preoperatively could significantly reduce the number of phospho-ERK positive neurons. ③Westem blot expression of phospho-ERK1/2 protein in the lumbar spinal cord increased signi ficantly 1 h after incision and decreased by intratheeal injection of SC-560. Conclusion： SC-560 administrated intrathecally can inhibit mechanical hypersensitivity induced by postoperative pain in rats and this anti-allodynic process may mediated by spinal ERK.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2007年第1期46-50,共5页
Chinese Journal of Applied Physiology
关键词
术后疼痛
脊髓
环氧合酶
细胞外信号调节激酶
postoperative pain
spinal cord
cyclooxygenase
extracellular signal-regulated kinase（ERK）
