摘要
利用游离小孢子培养获得的DH系群体和BSA法对与大白菜橘红心基因紧密连锁的分子标记进行了研究,筛选到与橘红心球色基因or连锁的RAPD标记S1338656和AFLP标记P67M54172,其遗传距离分别为8cM和13cM,并将其成功转化成SCAR标记SCOR204和SCOR127。对标记的通用性在110个大白菜自交不亲和系育种材料中进行了验证,与田间鉴定结果的吻合率分别为90%和89.1%。SCAR标记的获得为大白菜橘红心性状的分子标记辅助选择与基因的图位克隆奠定了基础。
Bulked segregant analysis was employed to identify molecular markers linked to orange head leaf gene （or） trait based on RAPD and AFLP in Chinese cabbage with a doubled haploid （DH） population derived from the F1 between the two parents 91-112 （white head leaf） and T12-19 （orange head leaf） via microspore culture. One RAPD marker S1338656 and one AFLP marker P67M54172 were identified to be linked to or gene with the distance of 8 cM and 13 cM respectively. These two markers were successfully transformed into SCAR markers SCOR204 and SCOR127. The accuracy of 90% and 89. 1% were obtained when 110 different incompatibility breeding lines of Chinese cabbage were used to be investigated with two SCAR markers, indicating that these two SCAR makers could be used in marker-assisted-selection in orange head leaf breeding programs and positional cloning of or gene in Chinese cabbage.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第1期217-220,共4页
Acta Horticulturae Sinica
基金
北京市自然科学基金项目(5062007)
国家自然科学基金项目(30671422)
国家‘863’项目(2006AA10Z1C9
2006AA100108)
关键词
大白菜
球叶色
BSA
SCAR
标记辅助选择
Chinese cabbage
Brassica rapa L. ssp. pekinensis
Orange head leaf gene （or）
BSA
SCAR
Marker-assisted selection
