摘要
以大百合的子房为外植体，研究了小鳞茎诱导及植株再生的方法。结果表明：BA和KT是影响大百合子房分化途径的关键因素，其浓度分别为0．1-1．0mg／L、2．0-4．0mg／L和高于4．0mg／L时，外植体分别分化为愈伤组织、芽和叶。外植体分化的基本培养基以N6、B5为佳。愈伤组织诱导小鳞茎的最佳培养基为MS＋0．1-0．5mg／LNAA＋2．5mg／LBA＋2．5mg／LKT＋10％蔗糖＋0．7％琼脂。在1／2MS＋3％蔗糖＋0．7％琼脂＋1％活性炭的生根培养基上，生根率为100％。炼苗一周后移栽，长势良好。
An experiment was carried out on the induction of bulbulets and the plant regeneration by using the ovaries of Cardiocrinum giganteum as explants. The results indicated that BA and KT were the main factors in ovary explant differentiation. The explants differentiated into calluses when the concentration of BA and KT was between 0. 1 and 1.0 mg/L and buds were induced when the concentration was bewteen 2. 0 and 4. 0 mg/L, and when higher than 4. 0 mg/L, leaves formed. The best basic media for the dedifferentiation were N6 and B5. The best medium for bulbulet induction from callus was MS ＋ 0. 1 - 0. 5 mg/L NAA ＋ 2. 5 mg/L BA ＋ KT 2. 5 mg/L ＋ 10% sucrose ＋0. 7% agar. The ratio of root induction from callus was 100% on the medium of 1/2MS ＋ 3% sucrose ＋ 0. 7% agar ＋ 1% active carbon. After acclimatization for one week, the bulblets were transplanted into greenhouse and all grew well.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第1期197-200,共4页
Acta Horticulturae Sinica
基金
国家科技部科技基础条件平台项目(2005DKA21006)
中国科学院创新方向性项目(KSCX2-SW-321)
关键词
大百合
组织培养
子房
Cardiocrinum giganteum
Tissue culture
Ovary
