摘要
目的研究次声对小鼠肾上腺皮质束状带多边形细胞酸性磷酸酶（acid phosphatase，ACP）、3-β羟甾脱氢酶（3-β hydroxysteroid dehydrogenase，3-βHSDH）的生物学效应。方法观察小鼠在频率8、16Hz，不同声压级80、90、100、110、120、130dB次声第1、7、14天暴露和停止暴露的14天后对肾上腺皮质束状带多边形细胞ACP、3-βHSDH的影响。结果单次1h暴露，8Hz组随声压级的升高。3-βHSDH活力增强，至130dB时，出现酶活力异常降低，同时提示100dB时出现多边形细胞损伤；16Hz组，80dB时小鼠3-βHSDH活力与对照组的差异无统计学意义（P〉0．05），但已提示存在细胞损伤，100dB时开始出现3-βHSDH活力增强，细胞损伤持续存在。持续次声暴露，8Hz中、低声压级随暴露时间延长。3-βHSDH活力先增强后降低，高声压级时酶活力持续为低水平，中、高声压级均提示出现细胞损伤；16Hz低声压级暴露表现同8Hz低声压级，中、高声压级3-βHSDH活力持续降低，同时提示存在细胞损伤。8Hz、90dB及130dB次声连续暴露14d后停止暴露，经14d间隔，多边形细胞表现为从有局部损伤迹象到有一定程度恢复，但原来暴露声压级越高，恢复程度越差。结论次声对小鼠肾上腺皮质束状带多边形细胞的生物学效应在一定作用强度范围内，可较明显地反映其频率作用特性；高强度、大剂量次声作用时，其特性常被严重的损伤效应所掩盖。脱离次声一段时间后，肾上腺皮质束状带的细胞有一定程度恢复，但原来暴露的声压级越高，恢复的程度越差。
Objective To explore the biological effects ofinfrasound on the polygonal cells in adrenal cortex zona fasciculatein in mice. Methods The biological effects of infrasound on the activities of 3-β hydroxysteroid dehydrogenase（3-βHSDH） and acid phosphatase（ACP） of the polygonal cells in adrenal cortex zona fasciculate were observed when exposed to 8 and 16 Hz infrasound at 80, 90, 100, 110, 120 and 130 dB for 1 day, 7 days and 14 days or 14 days after the exposure. Results When exposed to 8 Hz infrasound,the enzyme activities of 3-βHSDH increased as the sound pressure levels increased.Only when the sound pressure levels reached 130 dB,the enzyme activities began to decrease exceptionally.When exposed to 16 Hz,80 dB infrasound, no significant difference between the treatment and control group in the activities of 3-βHSDH could be observed, but the injury of the polygonal cells had appeared. When exposed to 16 Hz, 100 dB infrasound, the activities of 3-βHSDH started to increase.The cell injury still existed.When exposed to 16 Hz, 120 dB infrasound,the local tissue damage represented.Fourteen days after the mice were exposed to 8 Hz,90 dB and 130 dB infrasound for 14 days continuously,the local tissue injury of the adrenal cortex zona fasciculatein began to recover at certain extent,but the higher the exposure sound pressure level,the poorer the tissue recovery. Conclusion The biological effects of infrasound on the polygonal cells in adrenal cortex zona fasciculatein response to the frequency of the infrasound are found at certain action strength range,but this characteristic usually is covered by the severe tissue injury. When exposure to infrasound is stopped for a period of time,the local tissue injury of the adrenal cortex zona fasciculatein could recovers at certain extent,but the higher the exposure sound pressure level, the poorer the tissue recovery.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2007年第2期91-95,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
次声
肾上腺皮质
束状带
酸性磷酸酶
羟甾类脱氨酸
Infrasound
Adrenal cortex
Zona fasciculata
Acid phosphatase
Hydroxysteroid dehydrogenases
