摘要
使用MS附加2，4-D作基本培养基，对草地早熟禾进行愈伤组织的诱导和愈伤组织分化成苗试验，建立了最佳的早熟禾胚性愈伤诱导和植株再生体系．早熟禾的成熟种子经过灭菌后，接种到愈伤组织诱导培养基上，置于25±2℃，黑暗条件培养约8周-9周，有胚性愈伤组织产生．然后将愈伤组织转至分化培养基上，黑暗培养一周后，再进行光照培养，约20天后开始分化出根和芽，继续培养则长成粗壮的绿色植株．2，4-D是影响愈伤形成和植株再生的关键物质．本实验结果证明，3．0mg·L^-1和0．1mIg·L^-12，4．D是最佳浓度组合，其诱导频率和分化频率分别为67．82％和48．81％；0．5mg·L^-16-BA对根和芽的分化具有促进作用．
The optimal tissue culture system for embryonic callus induction and plant regeneration in blue grass has been set up by doing this experiment about callus induction and plant regeneration from mature seeds of Blue grass , which were cultured on MS basal medium supplemented with 2,4-D. Mature seeds of Blue grass were placed on autoclaved medium for callus induction after being sterilized. The cultures were incubated for 8 9 weeks at about 25℃ in darkness. Embryonic callus could be formed. Then some embryonic calli were transferred to differentiation medium for plant regeneration. These cultures for plant regeneration incubated for 1 week and then 20 days under fluorescent light （ 16/8h day/night）. Shoots and roots could be produced. Regeneration green plant could be received if continuous culture. 2,4-D is a kind of decisive ingredient for callus formation and plant regeneration. The results of this experiment have shown 3.0mg · L^ -1 and 0. 1mg · L^-1 2,4-D is the best consistency. The frequency of callus induction and frequency of differentiation are respectively 67.82% and 48.81%. 0.5mg · L^-1 6-BA can promote differentiation of roots and buds.
出处
《山西师范大学学报（自然科学版）》
2007年第1期99-102,共4页
Journal of Shanxi Normal University(Natural Science Edition)
关键词
早熟禾
胚性愈伤
植株再生体系
诱导频率
分化频率
bluegrass
embryonic callus
planflet regeneration system
frequency of induction
frequency of differentiation
