摘要
目的建立选择性外周血自细胞腺苷A2A受体基因缺失的小鼠模型。方法分别采用一次9．5GyX线照射和2次6．2GyX线间隔照射对小鼠进行清髓处理，将腺苷A2A受体基因敲除的小鼠骨髓细胞移植到清髓性处理的野生型小鼠体内，使其白细胞的腺苷A2A受体选择性缺失，并对移植效果进行鉴定。结果通过基因型鉴定发现骨髓移植6周后受体小鼠的白细胞性染色体基因PCR产物电泳条带为300和330bp；腺苷A2A受体阳性细胞率为10．21％，而野生型小鼠为96．72％；2次分割放疗结合大于6×10。个骨髓细胞的移植量可以得到满意的小鼠存活率（91％）。结论成功地建立了选择性缺失白细胞腺苷A2A受体基因的小鼠模型。
Objective To establish a mice model with selective inactivation adenosine A2A receptors （A2ARs） in peripheral white blood cells（PWBC）. Methods A2ARs were selectively inactivated in PWBCs by transplanting bone marrow cells（BMCs） from A2AR knockout （KO） mice into their wild type （WT） littermates after a single total body irradiation of 9.5 Gy or fractionated total body irradiation of 6.2 Gy × 2. The efficiency of reconstitution of bone marrow-derived cells in chimeric mice was assessed. Results PCR band patterns changed from the recipient pattern （ one band of 330 bp） to the donor （ two bands of 300 and 330 bp） pattern. Immunohistochemistry analysis showed that 10.21% of cells were A2AR^＋ in PWBCs in KO→WT mice, whereas 96.72% of cells were A2AR^＋ in WT mice. The survival rates of mice irradiated with 6.2 Gy×2 and transplanted with more than 6 × 10^6 BMCs were about 91%. Conclusion A murine model of selective inactivation adenosine A2A receptors in PWBCs was established successfully.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2007年第3期174-177,共4页
Chinese Journal of Hematology
基金
国家自然科学基金海外青年学者合作研究基金资助项目（30328015）.志谢：本研究工作得到美国波士顿大学神经科学系陈江帆教授的指导并提供腺苷A2A受体基因敲除鼠,特此致谢
关键词
白细胞
受体
腺苷A2A
骨髓移植
White blood cells
Receptors, A2A
Bone marrow transplantation
