摘要
目的:建立一种简单、较理想的新生大鼠皮层神经元体外原代培养方法。方法:采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液,进行培养。结果:在体外培养条件下神经元结构特征明显,并能形成典型的神经元网络。结论:本方法适合普通实验室进行大鼠皮层神经元体外培养。
Objective：To establish an easier and better primary culture technique suitable to neurons of newborn rat cortical tissure. Methods：Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture. Results：Under the culture condition in vitro,newborn rat neurons showed similar properties to their counterparts in vivo and typical nerver fiber net was formed. Conclusion：This technique for the primary culture of cortical neurons is an ideal good technique for the general labs.
出处
《重庆医科大学学报》
CAS
CSCD
2007年第4期392-394,401,共4页
Journal of Chongqing Medical University
