摘要
目的从灵芝Ganoderma lucidum子实体中分离得到的GL-PPT2、GL-PPT3和GL-PPT43种多糖肽,对其单糖组成和摩尔比进行比较。方法多糖肽经三氟乙酸水解后,用1-苯基-3-甲基-5-吡啶啉酮(PMP)衍生化,在高效液相245nm紫外检测,使用KH2PO4(pH5.0)-乙腈(83.5∶16.5)等度洗脱,9种单糖的色谱峰分离清晰,大多呈基线分离。结果经实验确定GL-PPT2、GL-PPT3和GL-PPT4均由甘露糖、核糖、鼠李糖、葡萄糖、半乳糖、阿拉伯糖、木糖、岩藻糖和葡萄糖醛酸等9种单糖组成。结论灵芝子实体活性成分多糖肽中的核糖系属首次报道。
Objective To compare the monosaccharide composition and molar ratio of GL-PPT2, GL- PPT3, and GL-PPT4, which are three polysaccharide peptides isolated from the fruit body of Ganoderma lucidum. Methods The polysaccharide peptides were hydrolyzed by triflouroacetic acid, then derivatized by trichloro-aldehyde-l-pheny-3-methyl-5-pyrazolone （PMP）, and determined by HPLC method with the 245 nm detection wavelength. Gradient elution with the solution of KH2PO4（pH 5.0）-acetonitrile （83.5 ： 16.5） resulted in baseline separation of nine monosaccharides, of which the chromatographic peaks were very clear. Results GL-PPT2, GL-PPT3, and GL-PPT4 comprise the nine monosaccharides of mannose, ribose, rhamnose, glucose, galactose, arabinose, xylose, galactose, and glucuronic acid. Conclusion The ribose in polysaccharide peptides of G. lucidum is first reported.
出处
《中草药》
CAS
CSCD
北大核心
2007年第2期174-176,共3页
Chinese Traditional and Herbal Drugs
基金
上海绿谷集团有限公司科研基金资助项目
关键词
灵芝
灵芝多糖肽
单糖
Ganoderma lucidum （Leyss. ex Fr. ） Karst.
Ganoderma lucidum polysaccharide peptides
monosaccharides
