摘要
目的:分析中日友好医院血培养中病原菌的构成与耐药情况,明确血液感染的病原菌分布和耐药模式,为合理使用抗菌药物提供参考。方法:应用WHONET 5.3进行耐药率统计;采用微量肉汤稀释法测定最低抑菌浓度;PCR检测葡萄球菌mecA基因。结果:革兰阳性球菌比革兰阴性杆菌分离率高。mecA基因阳性率在金黄色葡萄球菌和凝固酶阴性葡萄球菌中分别为32.3%和95.8%;革兰阴性杆菌中分离率较高的是大肠埃希菌、肺炎克雷伯菌和鲍曼不动杆菌。超广谱-β内酰胺酶(ESBLs)菌株分别占大肠埃希菌和肺炎克雷伯菌的10.4%和11.2%。结论:血培养苯唑西林耐药葡萄球菌菌株分离比例较高,ESBLs菌株在大肠埃希菌和肺炎克雷伯菌中占一定比例。
Objective：To provide guide for reasonable antimicrobial therapy of pathogenic microorganisms from blood culture and their antimicrobial resistance.Methods：Minimal inhibitory concentration was determined with micro broth dilution and WHONET 5.3 was applied to summarize resistant,intermediate and susceptible.mecA was detected With PCR in staphylococci.Results：The separating rate of gram-positive coccus was higher than that of gram-negative bacillus.mecA detected in S.aureus and coagulase negative staphylococcus was 32.3% and 95.8% respectively.The separating rate was high in E.coli,K.pneumoniae and A.bauamnnii.ESBLs in E.coli and K.pneumoniae were 10.4% and 11.2%.Conclusion：methicillin resistant staphylococcus is of high percentage in staphylococcus and ESBLs is of certain percentage in E.coli and K.pneumoniae from the blood culture.
出处
《中日友好医院学报》
2007年第1期15-18,共4页
Journal of China-Japan Friendship Hospital
基金
首都医学发展科研基金(2002-2019)
关键词
病原菌
血培养
耐药性
pathogenic microorganism
blood culture
antimicrobial resistance
