摘要
目的建立反相高效液相色谱法测定妥布霉素滴眼液含量的方法。方法选用C18色谱柱，2，4-二硝基氟苯（DNFB）柱前衍生化，0.25％三羟甲氨基甲烷-乙腈-0．5mol·L^-1硫酸溶液（40：59：1）为流动相，检测波长为365nm。结果在10．56-15．84μg范围内峰面积与浓度呈良好线性关系，r=0．9996；平均回收率为99，90％。RSD为0．37％。结论该方法简便准确，重现性好，可用于妥布霉素滴眼液含量的测定。
Objective To determine the content of tobramycin in eye drops by RP-HPLC. Methods The precolumn derivation using 2,4-dinitroflurobenzene（DNFB） was carried out. The chromatographic separation was performed in C18 column. The mobile phase was 0, 25% tris（hydroxymethyl） aminomethane-ancetonitrile-0. 5 mol·L^-1 sulfuric acid（40： 59： 1 ）. The detection wave length was 365 nm. Results The linear range was 10. 56 - 15. 84 μg （ r = 0. 999 6）. The average recovery was 99.90% （RSD = 0. 37% ）. Conclusion The method was simpl, accurate and highly reproducible , and can be used for the quality control of tobramycin in eye drops.
出处
《医药导报》
CAS
2007年第3期291-292,共2页
Herald of Medicine
