摘要
目的:研究脓毒血症时肾脏组织细胞凋亡的变化。方法:通过对SD大鼠盲肠结扎及贯穿,建立大鼠的脓毒血症模型,取肾组织制成组织匀浆液。细胞凋亡使用PI/Annexin V(碘化丙啶/磷脂结合蛋白V)双染色法,用流式细胞仪测定,及通过末端标记(Tunel)技术免疫组织化学法分析细胞凋亡。结果:在SD大鼠脓毒症早期,肾组织细胞凋亡数增加。结论:脓毒血症早期肾组织细胞凋亡数量增加,与细胞因子大量释放有关。
Objective：To investigate the changes of renal cell apoptosis in SD rats with sepsis. Methods： Model rats with sepsis was established by cecum ligation and puncture Renal tissue homogenate was prepared. Apoptosis was analyzed by PI/AnnexinV by flow cytometer and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling （tuned technique Results： The number of renal cell apoptosis increased in the early stage of sepsis. Conclusions： The number of renal cell apoptosis increased in the early stage of sepsis, and it correlated with the releasing of cytokines.
出处
《内科急危重症杂志》
2007年第1期28-29,32,共3页
Journal of Critical Care In Internal Medicine
