摘要
目的研究脓毒症时肾脏组织细胞凋亡变化与炎症细胞因子的关系。方法采用盲肠结扎穿孔术(CLP)建立SD大鼠脓毒症模型。分别取肾组织,通过组织裂解液,制备组织匀浆,测定匀浆上清中肿瘤坏死因子α(TNFα)、单核细胞趋化蛋白1(M CP 1)含量;采用钙结合蛋白V/碘化丙啶(A nnex in V/P I)双染色法和流式细胞仪测定组织凋亡细胞,采用原位末端缺刻标记技术(TUNEL)和免疫组化分析细胞凋亡情况。结果在脓毒症早期,肾组织匀浆中TNFα、M CP 1含量均明显增高,肾组织细胞凋亡数量增加以致伤后3 h为最高,6 h后逐渐下降,差异均有显著性(P<0.05或P<0.01)。结论TNFα、M CP 1是脓毒症早期激活的细胞因子,脓毒症早期肾组织细胞凋亡数量增加与细胞因子大量释放有关。
Objective To investigate the relationship between changes in renal cell apopmsis and inflammatory cytokines in sepsis rats. Methods Sepsis was produced in rats by cecal ligation and puncture. Changes in tumor necrosis factor -α （TNF-α） and monocyte chemotactic protein - 1 （MCP - 1 ） levels in renal tissues were determined by enzyme - linked immunoadsorbent assay （ELISA）, respectively. Meanwhile, apoptosis rate was also assessed by Annexin V/PI staining and flow cytometry and terminal deoxynucleotidyl transferase -mediated dUTP biotin nick end labeling （TUNEL） methods, Results TNF-α and MCP - 1 levels in renal tissues were significantly increased in rats with sepsis in the early stage. Apoptosis rate of renal cells increased and reached the highest level at 3 hours after sepsis was established and decreased after 6 hours, and the differences were significant （P〈0.05 or P〈0.01 ）. Conclusion TNF-αand MCP - 1 are cytokines which are activated in early stage of sepsis. The increase in renal cell apoptosis in the early stage of sepsis is related with the release of cytokines,
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2006年第2期89-91,I0001,共4页
Chinese Critical Care Medicine
基金
辽宁省教育厅科研基金资助项目(2004D124)
