摘要
采用一种体外培养的人小肠上皮细胞模型Caco-2研究了时间、浓度、温度对氯化铬和吡啶羧酸铬细胞摄取和跨细胞转运的影响。旨在探讨氯化铬和吡啶羧酸铬在小肠上皮细胞中的摄取和转运特点。结果表明:Caco-2细胞对氯化铬和吡啶羧酸铬的摄取和转运随浓度、时间而成线性增加,当温育温度从37℃降到4℃时,摄取和转运有下降趋势(P>0.05)。氯化铬和吡啶羧酸铬从肠腔侧(AP侧)到基底侧(BL侧)的表观通透系数(Papp)近似于BL侧到AP侧(分别为0.95～1.41倍和0.84～1.07倍)。氯化铬和吡啶羧酸铬的摄取率分别为(0.88±0.08)%和(4.73±0.60)%,转运率分别为(2.11±0.05)%和(9.08±0.25)%。结果提示氯化铬和吡啶羧酸铬是以被动扩散为主要方式被Caco-2细胞摄取和转运。
In order to investigate the uptake and epithelial transport characteristics of chromium chloride and chromium picolinate in small intestine, a human intestinal epithelial cell model Caco-2 in vitro cultured was used to study the effects of time, concentration and temperature on uptake and transport of chromium chloride and chromium picolinate. The results showed that the uptake and transport of chromium chloride and chromium picolinate were increased linearly with the increase of concentration and incubation time, and were inclined to be decreased （P〉0.05） when the incubation temperature was lowered from 37 ℃ to 4 ℃. The apparent permeability coefficient （Papp） of chromium chloride and chromium picolinate from apical to basolateral was similar to that from basolateral to apical （0.95-1.41 fold and 0.84-1.07 fold respectively）. The uptake rate of chromium chloride and chromium picolinate was （0.88±0.08）% and （4.73±0.60）%, respectively. The transport rate of chromium chloride and chromium picolinate was （2.11±0.05）% and （9.08±0.25）%, respectively. Thus, the uptake and transport of chromium chloride and chromium picolinate were passive diffusion as the dominating process.
出处
《细胞生物学杂志》
CSCD
2007年第1期77-80,共4页
Chinese Journal of Cell Biology
基金
浙江省自然科学基金项目资助(No.M303450)~~
