摘要
使用DNA断端标记法（TDT法）定量观察了白血病细胞株HL－60和U937对顺氯氨铂，羟基喜树碱，长春新碱3种抗肿瘤药物所发生的凋亡变化。HL－60对顺氯氨铂很敏感。U937对羟基树碱、长春新碱敏感。比较了核酸电泳检测的结果。并首次报道了用TDT法在荧光显微镜下可观察到细胞凋亡的三种不同形态。
DNA strand breaks which occur in HL-60 and U937 cells as a result of apoptosis inducedby co-cultured with three antineoplasm drugs were studied. The drugs used were cis-diamin-odichloroplatinum,hydroxycamptothecinum and vindesini sulfa. The DNA strands were la-beled in situ with biotinylated dUTP, using the terminal deoxynucleotidyl transferase (TDTin situ), which was detected by fluoresceinated avidin. HL-60 and U937 showed differentsensitivity to these reagents. Under UV microscopy and flow-cytometry and observation ofapoptosis percentage and apoptotic morphology of both cell lines, three types of apoptosiswere first detected by TDT in situ.
出处
《肿瘤》
CAS
CSCD
北大核心
1996年第3期391-394,共4页
Tumor
基金
国家自然科学青年基金!39400179
