摘要
采用水相法合成的CdTe半导体纳米粒子作为能量给体,通过Sch iff碱反应将单链DNA连接到表面.采用柠檬酸钠还原氯金酸法制取的Au纳米粒子作为能量受体,通过Au—S键将单链DNA连接到表面.通过DNA链间的杂交,构建了荧光共振能量转移体系(FRET).测定了CdTe-DNA、探针体系和探针体系+目标DNA的荧光强度.结果表明,探针体系的荧光强度最弱,加入目标DNA后,体系荧光增强,表明该体系的构建是成功的.
CdTe semiconductor nanoparticles which were synthesized in water phase were taken as the energy donors. CdTe nanoparticles were linked to 5＇-NH2-DNA by Schiff＇s reaction. Au nanoparticles which were prepared by sodium citrate reduction of chloroauric acids were taken as the energy acceptors. Au nanoparticles were linked to 3＇-SH-DNA by Au-S bond. The fluoresscence resonance energy transfer（FRET） probes are generated through hybridization of complementary DNA bound to the QDs and Au nanoparticles. The fluorescence spectra of CdTe-DNA, CdTe-DNA-Au and CdTe-DNA-Au ＋ target DNA were measured. The data show that the fluorescence intensity of CdTe-DNA-Au conjugates was the weakest. When adding a complementary ssDNA to CdTe-DNA-Au conjugates, the fluorescence intensity of the system increased. This result shows that the configuration of FRET was successful.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2007年第2期254-257,共4页
Chemical Journal of Chinese Universities
基金
天津市科委科学基金(批准号:043612711)资助
关键词
金纳米粒子
CdTe纳米粒子
荧光共振能量转移
荧光猝灭
Au nanoparticle
CdTe nanoparticle
Fluorescence resonance energy transfer （FRET）
Fluorescence quenching
