摘要
为了促进亚洲百合分子水平的研究,以亚洲百合幼嫩叶片为材料,采用改良CTAB法提取亚洲百合基因组DNA,得到的DNA质量较高,适合于RAPD-PCR分析。通过单因素优化得到在25μl反应体系中,亚洲百合RAPD分析的最佳反应体系:160μmol/LdNTPs,0.3μmol/L随机引物,TaqDNA聚合酶1.5U,Mg2+浓度2.0mmol/L。
In order to accelerate the molecular biology research of Lilium asiatic hybrids, DNA of Lilium asiatic hybrids was extracted from the fresh leaves by CTAB. The optimized reaction system for Lilium asiatic hybrids are as follows. The reactions were performed in a volume of 25 μl containing 2.0 mmol/L Mg^2＋, 160μmol/L dNTPs, 1.5 U Taq DNApolymerase, 0.3 μmol/L primer.
出处
《中国农学通报》
CSCD
2007年第2期89-92,共4页
Chinese Agricultural Science Bulletin
