4OhashiR,MochizukiE,KamoshitaY.High-level expression of the methan61-inducible beta-galactosidase gene by perfusion culture of recombinant Pichia pastoris using a shaken ceramic membrane flask[J].Journal of Fermentation & Bioengineering,1998,86 (1):44 ~48.
5Villatte F,Hussein AS,BachmanoTT.Expression level of heterologous proteins in Pichia Pastoris is influenced by flask design[J].Appl Microbiol Biotechnol 2001,55:463~465.
6Wood M J,Komives E A.Production of large quantities of isotopically labeled proteinin Pichia Pastoris by fermentation[J].Journal of Biomolecular NMR,1999,13(2):149~159.
7Thorpe E D,Anjou M c,Daugul is A J Sorbitol as a non-repressing carbon source forfed-batch fermentation of recombinant Pichia pastoric[J] Biotechnology Letters,1999,21 (8):609~ 672.
8Ohashi R,Mochizuki E,Suzuki T.A mini-scale mass production and separation systemfor secretory heterologous proteins by perfusion culture of recombinant Pichiapastoris using a shaken ceramic membrane flask[J]Journal of Bioscience&Bioengineerlng,1999,87(5):655~660.
9Hellwig s,Erode LRaven NP,et al.Analysis of single-chain antibody production in Pichiapastoris using On-line methanol control in fedbatch and mixed-feed fermentations[J].Biotechnol Bioeng,2001,74(4):344.
10lnan M,Meagher MM.The effect of ethanol and acetate on protein expressing[J]J Bioscoi Bioeng,2001,92:329.
3Sambrook J Fritsch E F.分子克隆实验指南(第二版)[M].北京:科学出版社,1992..
4金冬雁(译),分子克隆实验指南(第2版),1992年
5赵翔,复旦学报,1999年,38卷,5期,510页
6GREGG J M, TSCHOPP J F, STILLMAN C, et al. High-level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methy Lotrophic Yeast Pichia Pastoris[J]. Bio/Technology, 1987, (5):479-486.
7MC GREW J T, LEISKE D, DELL B, et al. Expression of Trimeric CD40 Ligand in Pichia Pastoris: Use of a Rapid Method to Detect High-level Expression Trarsformants[ J]. Gene, 1997, ( 187 ): 193-198.
8CLARE J J, ROMANOS M A. Production of EGF in Yeast:High-level Secretion Using Pichia Pastoris Strains Containing Multiple Gene Copies[J]. Gene, 1991,105:205-212.
9MEI M, WHITTAKER, JAMESW, et al. Expression of Recombinant Galactose Oxidase by Pichia Pastoris[ J]. Protein Expression and Purification,2000,20:105-111.
10CREGG J M,UDEVICK T S, RASCHKE W C. Recent Advances in the Expression of Foreign Genes in Pichia Pastoris[J]. Bio/Technology, 1993,11 (8) :905-910.