摘要
目的 探讨亚低温和高温对内毒素致急性肺损伤（Au）大鼠肺微血管通透性的影响。方法 健康雄性SD大鼠48只，体重300～350g，随机分为4组（n=12），常温对照组（A组）静脉注射37℃生理盐水；常温内毒素组（B组）静脉注射脂多糖（LPS）5mg·kg^-1；亚低温＋内毒素组（C组）静脉注射LPS5mg·kg^-1，维持直肠温31．5～32％；高温＋内毒素组（D组）静脉注射LPS5mg·kg^-1，维持直肠温39．5～40℃。动脉血氧合指数（PaO2／FiO2）≤300mmHg即ALI模型制备成功。于Au6h后处死大鼠，取肺组织，清洗固定后测定肺微血管通透性指标：肺湿／干重量比（W／D）、肺泡灌洗液（BALF）蛋白（BALFpro）、肺通透性指数（PPI）、肺微血管通透性指数（PMPI）及肺组织硝基酪氨酸（NT）、髓过氧化物酶（MPO）、丙二醛（MDA）含量，观察肺组织病理学改变。结果 与A组比较，B组、C组、D组PPI、W／D、BALFpro、PMPI和肺组织MPO、MDA、NT均升高（P〈0．05）；与B组比较，C组上述指标降低，D组上述指标升高（P〈0．05）；与C组比较，D组PPI、W／D、BALFpro、PMPI和肺组织MPO、MDA、NT升高（P〈0．05）。结论 高温加重内毒素血症大鼠ALI程度，亚低温通过抑制ONOO^-生成，抑制了肺微血管通透性的升高，可减轻大鼠内毒素致ALI。
Objective To investigate the effects of mild hypothermia and hyperthermia on endotoxininduced acute lung injury （ALI）.Methods Forty-eight male SD rats weighing 300-350 g were randomly divided into 4 groups （ n = 12 each） ： group A normothermia ＋ normal saline; group B normothermia ＋ LPS （5mg·kg^-1 ） ; group C hypothermia （31.5-32℃） ＋ LPS （5 mg·kg^-1） and group D hyperthermia （39.5～40℃） ＋ LPS （5mg·kg^-1）. ALI was considered established when PaO2/FiO2 ≤300 mm Hg. The animals were killed by blood-letting. The lungs were removed for microscopic examination and for determination of W/D lung weight ratio, protein content of broncho-alveolar lavage fluid （BALF） , pulmonary permeability index （PPI） , pulmonary mierovaseular permeability index （PMPI）, myeloperoxidase （MPO） activity, and nitrotyrosine （NT） and malondialdehyde （MDA） content. Results The W/D lung weight ratio, protein content of BALF, MPO activity, MDA and NT content, PPI and PMPI were significantly increased by IV LPS administration in group B, C and D as compared with group A. The histologic damage to the lung and the pulmonary variables were significantly decreased in group C and increased in group D as compared with group B （P 〈 0.05 ）. Conclusions Mild hypothermia has protective effect on the lung against endotoxin-induced injury while hyperthermia aggravates ALI.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2007年第1期75-78,共4页
Chinese Journal of Anesthesiology
关键词
体温
内毒素血症
呼吸窘迫综合征
成人
毛细血管通透性
Body temperature
Endotoxemia
Respiratory distress syndrome, adult
Capillary permeability
