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CO_2对人卵巢癌细胞SKOV3转移因子表达的影响

Effects of carbon dioxide on the expression of metastatic factors in human ovarian carcinoma cell line SKOV3
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摘要 目的:探讨二氧化碳(CO_2)对卵巢恶性肿瘤细胞转移能力的影响。方法:体外培养人卵巢浆液性乳头状腺癌SKOV3细胞株,分为CO_2组、N_2组和对照组,分别在CO_2及N_2处理后2h,置于常规条件下培养12、24、48h通过RT-PCR和Western Blot检测不同时相血管内皮生长因子C(VEGF-C)和基质金属蛋白酶9(MMP9)的表达。结果:CO_2处理后,SKOV3细胞VEGF-C和MMP9的mRNA和蛋白质表达显著增加(P＜0．05);N_2处理后,SKOV3细胞VEGF-C和MMP9的mRNA和蛋白质表达较对照组增加(P＜0．05)。结论:CO_2促进了SKOV3 VEGF-C和MMP9的表达,对SKOV3的转移能力有增强作用。 Objective ：To study the effects of carbon dioxide on the expression of metastatic factors in ovarian carcinoma cell line SKOV3. Method：Ovarian carcinoma cell line SKOV3 was treated with 100% carbon dioxide for 2 hours,then put into normal circumstance for 12hours,24hours,48hours,respectively;Examine the expressions of different times of VEGF-C and MMP9 by RT-PCR and Western Blot. Result： After the treatment of carbon dioxide, the expression of VEGF-C and MMP9 significantly increased compared with controlled groups. Conclusion：Carbon dioxide promotes the expression of VEGF-C and MMP9 in SKOV3, so it may improve the metastatic capability of ovarian carcinoma cell line.
作者 黄金 唐均英 姚珍薇
机构地区 重庆医科大学附属第一医院妇产科
出处 《现代妇产科进展》 CSCD 北大核心 2007年第1期29-32,共4页 Progress in Obstetrics and Gynecology
关键词 卵巢肿瘤 二氧化碳 细胞株SKOV3 血管内皮生长因子-C 基质金属蛋白酶-9 Ovarian neoplasms Carbon dioxide Cell line SKOV3 Vascular endothelial growth factor-C Matrix metalloproteinase-9
分类号 R737.3 [医药卫生—肿瘤]
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参考文献14

  • 1Jacobi CA,Bonjer HJ,Puttick Mi,et al.Oncologic implications of laparoscopic and open surgery[J].Surg Endosc,2002,16:441-445
  • 2Neuhaus SJ,Texler M,Hewett PJ,et al.Port-site metastases following laparoscopic surgery[J].Br J Surg,1998 Jun,85:735-741
  • 3Curet MJ.Port site metastases[J].Am J Surg,2004,187:705-712
  • 4Kruitwagen RF,Swinkels BM,Keyser KG,et al.Incidence and effect on survival of abdominal wall metastases at trocar or puncture sites following laparoscopy or paracentesis in women with ovarian cancer[J].Gynecol Oncol,1996,60:233-237
  • 5Ramirez PT,Wolf JK,Levenback C.Laparoscopic port-site metastases:etiology and prevention[J].Gynecol Oncol,2003,91:179-189
  • 6Volz J,Koster S,Spacek Z,et al.The influence of pneumoperitoneum used in laparoscopic surgery on an intraabdominal tumor growth[J].Cancer,1999,86:770-774
  • 7黄锐,刘彦,金志军,陈旭,李文.气腹对裸鼠腹腔恶性肿瘤细胞种植和生长的影响[J].第二军医大学学报,2003,24(3):285-287. 被引量：8
  • 8Hopkins MP,Dulai RM,Occhino A,et al.The effects of carbon dioxide pneumoperitoneum on seeding of tumor in port sites in a rat model[J].Am J Obstet Gynecol,1999,181:1329-1333
  • 9Kuntz C,Wunsch A,Bodeker C,et al.Effect of pressure and gas type on intraabdominal,subcutaneous,and blood pH in laparoscopy[J].Surg Endosc,2000,14:367-371
  • 10Jeltsch M,Kaipainen A,Joukou V,et al.Hyperplasia of lymphatic vessels in VEGF-C transgenic mice[J].Science,1997,276:1423-1425

二级参考文献36

  • 1Hopkins M P, Dulai R M, Occhino A, et al. The effects of carbon dioxide pneumoperitoneum on seeding of tumor in port sites in a rat model[J]. Am J Obstet Gynecol, 1999, 181(6): 1329 -1333.
  • 2Kuntz C, Wunsch A, Bodeker C, et al. Effect of pressure and gas type on intraabdominal, subcutaneous, and blood pH in laparoscopy [ J ].Surg Endosc, 2000, 14(4): 367 -371.
  • 3Hicklin D J, Ellis L M. Role of the vascular endothelial growth factor pathway in tumor growth and angiogenesis [ J ]. J Clin Oncol, 2005,23(5): 1011 -1027.
  • 4Nishida N, Yano H, Komai K, et al. Vascular endothelial growth factor C and vascular endothelial growth factor receptor 2 are related closely to the prognosis of patients with ovarian carcinoma[ J]. Cancer, 2004, 101(6): 1364 -1374.
  • 5Jacobi C A, Bonjer H J, Puttick M I, et al. Oncologic implications of laparoscopic and open surgery[J]. Surg Endosc, 2002, 16(3): 441- 445.
  • 6Neuhaus S J, Texler M, Hewett P J, et al. Port-site metastases following laparoscopic surgery[J]. Br J Surg, 1998, 85(6): 735 -741.
  • 7Curet M J. Port site metastases[J]. Am J Surg, 2004, 187(6): 705-712.
  • 8Kruitwagen R F, Swinkels B M, Keyser K G, et al. Incidence and effect on survival of abdominal wall metastases at trocar or puncture sites following laparoscopy or paracentesis in women with ovarian cancer[J]. Gynecol Oncol, 1996, 60(2): 233 -237.
  • 9Ramirez P T, Wolf J K, Levenback C. Laparoscopic port-site metastases: etiology and prevention[J]. Gynecol Oncol, 2003, 91 ( 1 ): 179- 189.
  • 10Volz J, Koster S, Spacek Z, et al. The influence of pneumoperitoneum used in laparoscopic surgery on an intraabdominal tumor growth [ J ].Cancer, 1999, 86(5): 770 -774.

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