摘要
目的探讨三氧化二砷(As2O3)抑制人胃癌细胞株MKN-28生长及诱导其凋亡的生物学效应。方法采用光镜观察、溴化二甲噻唑二苯四氮唑(MTT)还原法、流式细胞仪(FCM)检测法、琼脂糖DNA凝胶电泳法检测As2O3在不同作用时间、不同给药浓度时对MKN-28细胞生长的影响。结果MKN-28细胞经As2O3作用后,细胞增殖受到明显抑制,而且呈浓度-作用时间依赖关系。流式细胞仪检测结果显示DNA直方图上出现典型的亚二倍体凋亡峰,细胞周期分析发现药物作用后MKN-28细胞的生长周期受到明显阻滞。琼脂糖DNA凝胶电泳检测到细胞发生凋亡时由于DNA的规律性降解而形成的梯状条带。结论As2O3既能有效地抑制人胃癌细胞株MKN-28的生长,又能诱导该细胞凋亡,而且呈浓度依赖性和作用时间依赖性。
Objective To study the biological effects of arsenic trioxide （As2O3） on human gastric cancer cells MKN-28 in vitro. Methods By using light microscopy, MTT reduction assay, flow cytometry and DNA agarose gel electrophores, the apoptosis of human gastric cancer cells treated with different concentrations of As2O3 was detected during different periods. Results As2O3 could significantly inhibit the proliferation of MKN-28 cells in a dose- and time-dependent manner. The cells treated with As2O3 showed a typical apoptotic morphology and hypodiploid peak before G1 phase. Prominent apoptotic morphological change was examined by DNA agarose gel electrophores. Conclusion As2O3 not only could inhibit significantly the proliferation of MKN-28 cells but also could induce apoptosis of MKN-28 cells.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期120-123,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词
三氧化二砷
胃癌细胞
细胞凋亡
arsenic trioxide
gastric cancer cells
apoptosis
