摘要
研究了在植物检疫中利用蚧虫残体提取DNA并应用PCR技术快速鉴定虫种的方法。结果表明,该方法对蚧虫残体DNA的提取效果很好,并筛选出了适合蚧虫DNA扩增的引物“AGAGGTGGGCAGGTG”;利用该引物对枣大球蚧(Eulecanium gigantea Shinji)、朝鲜毛球蚧(Didesmococcus koreanus Borchsenius)、日本龟蜡蚧(Ceroplastes japonicus Green)和白蜡绵粉蚧(Phenacoccus fraxinus Tang)4种蚧虫进行PCR扩增后均有很好的特异性,这为蚧虫的快速检疫鉴定提供了新途径。
A new method of total DNA extraction from the incomplete body of scale insects and PCR technique for rapid identification of species in plant quarantine was studied. The results showed that the method of DNA extraction from incomplete body of scale insect was very effective. The primer ＂AGAGGTGGGCAGGTG＂ to DNA PCR amplification of scale insect was selected. By using this primer, four species of scale insect showed great specificity after PCR amplification, which were Eulecanium gigantea Shinji, Didesmococcus koreanus Borchsenius, Ceroplastes japonicus Green and Phenacoccus fraxinus Tang. Therefore, the results confirmed that this method was a new way of scale insect rapid identification in plant quarantine.
出处
《安徽农业科学》
CAS
北大核心
2007年第5期1302-1304,共3页
Journal of Anhui Agricultural Sciences
关键词
蚧虫
PCR
检疫
快速鉴定
Scale insect
PCR technique
Quarantine
Rapid identification
