摘要
为了提高马铃薯对细菌性病害的抗性,以来源于膜翅目昆虫的新多肽抗生素Apidaecin为目的基因,以多肽抗生素Shiva-Ⅰ为对照,对多肽抗生素在马铃薯抗青枯病基因工程中的应用进行了研究。采用农杆菌介导的方法,用含有Apidaecin+Shiva-Ⅰ双价基因的植物表达载体pBinPRSIHbI对马铃薯栽培品种米拉、中-5-1、Favorata和尼勒克进行了遗传转化试验,共获得卡那抗性再生植株31个,获得了最佳的植株再生分化体系和遗传转化条件。PCR检测及RT-PCR分析表明,目的基因已成功地整合到转基因植株的染色体上并在转基因马铃薯中进行了转录。
In order to enhance the disease resistance of potato to bacterial diseases, peptide antibiotic （Apidaecin） from honeybee, was selected to study its feasibility in engineering potato for bacterial disease resistances, pBinPRSIHbI with another peptide antibiotic Shiva-I as control, a bivalent plant expres- sion vector containing Apidaecin and Shiva-I genes, was introduced into potato cultivars Mira, Zhong-5- 1, Favorata and Nileke via Agrobacterium tumefaciens-mediated transformation. Thirty-one Kanamycin resistant regenerated seedlings were obtained. A high efficient regeneration system has been established in potato. Results from PCR and RT-PCR analysis indicated that foreign genes were successfully integrated into the genome and transcribed in transgenic potato.
出处
《植物保护学报》
CAS
CSCD
北大核心
2007年第1期46-50,共5页
Journal of Plant Protection
基金
国家自然科学基金(30270872)
