摘要
目的:研究肿瘤坏死因子-α(TNF-α)对体外大鼠心脏致室性心律失常的作用,初步探讨致室性心律失常的机制。方法:采用大鼠体外心脏灌流模型观察TNF-α对心脏室性心律失常的作用,通过激光共聚焦技术观察TNF-α对单个分离心肌细胞钙浓度的影响。结果:不同浓度的TNF-α灌流能引起体外心脏室性心律失常,且室性心律失常发生次数随着TNF-α浓度的增加而增多(P<0.05);经TNF-α受体抗体预处理后同等浓度的TNF-α引起的室性心律失常较前明显减少(P<0.05)。200kU/LTNF-α可使心肌胞内钙荧光强度增加(121.0±13.3)%;TNF-α受体抗体预处理,TNF-α仅能使胞内钙荧光强度增加[(13.5±3.7)%,P<0.01]。结论:TNF-α能够引起体外心脏室性心律失常,可能与TNF-α使心肌胞内钙短时间大量增加有关。
Objective,To study the effects of tumor necrosis factor-α（TNF-α）on ventricular arrhythmias of isolated rat heart. Method： The isolated rat hearts were perfused with Krebs-Henseleit （K-H）solution via aorta at 37℃. Different concentrations of TNF-α were added to observe the arrhythmia. The effect of TNF-α on intracellular Ca^2＋ concentration of cardiac myocytes were detected by laser confocal technique. Result：Different concentrations of TNF-α could caused ventricular arrhythmias. The frequency of ventricular arrhythmias was interrelated with the concentrations of TNF-α（P〈0.05）. Using the antibody against TNF-α receptor beforehand, arrhythmias by TNF-α were less （P 〈 0.05）. TNF-α increased the ICa-L peak current amplitude and intracellular Ca^2＋ fluorescent intensity. Using the antibody against TNF-α receptor beforehand, TNF-α induced a slight increase of ICa-L peak current amplitude and a mild elevation of intracellular Ca^2＋ fluorescent intensity（P〈0. 01）. Conclusion：TNF-α causes ventricular arrhythmias on isolated rat hearts. This effect may be associated with the increased intracellular Ca^2＋ intensity caused by TNF-α.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2007年第2期141-143,共3页
Journal of Clinical Cardiology
