摘要
目的:观察口腔鳞癌细胞系(Tca8113和BcaCD885)对口腔癌靶向顺铂聚乳酸聚乙二醇纳米粒(CDDP-PLA-PEG-NP,或称隐形聚乳酸纳米)的敏感性,并与顺铂(CDDP)对Tca8113和BcaCD885的抗癌活性进行对比观察。方法:用四唑盐显色法(MTT法)检测CDDP-PLA-PEG-NP和CDDP在1-8 d内8个时间点对Tca8113和BcaCD885的抗癌活性,计算出2种药物在8个时间点对2种癌细胞的抑癌率和药物的半数抑癌率浓度。结果:CDDP-PLA-PEG-NP和CDDP对Tca8113和BcaCD885均有强的杀伤作用,其杀伤效应均呈时间依赖性和剂量依赖性。结论:经改型后的CDDP-PLA-PEG-NP对口腔鳞癌细胞Tca8113和BcaCD885仍有强大的杀伤作用,为以后进一步的体内实验提供了依据。
Objective： To study chemosensitivity and anticancer activity of Cisplatin （CDDP） loaded polylactic acid - polyethylene glycol nanoparticles （CDDP- PLA- PEG- NP） against oral squamous carcinoma cell lines Tca8113 （tongue carcinoma） and BcaCD885 （buccal carcinoma）. Methods： The chemosensitivity of Tca8113 and BcaCD885 to CDDP- PLA -PEG- NP and CDDP was tested using MTT assay. The suppressed cancer rate and 50% inhibition concentrations values （IC50） of two drugs against two cancer cell lines were counted for 1,2,3,4,5,6,7and 8 days at different concentrations. Results： Both CDDP -PLA -PEG -NP and CDDP showed high anticancer effects against Tca8113 and BcaCD855, the anticancer activity of two drugs was both dose and time dependent. Conclusion： CDDP within CDDP - PLA - PEG - NP remained its anticancer effects. And CDDP - PLA - PEG - NP showed higher than that of CDDP.
出处
《口腔医学研究》
CAS
CSCD
2007年第1期47-49,共3页
Journal of Oral Science Research
基金
重庆市科委应用基础研究项目(渝科发计字〔2003〕43号)
重庆市卫生局科研项目(2001)
关键词
口腔癌
聚乳酸
聚乙二醇
纳米粒
顺铂
Oral carcinoma Polylactic acid Polyethylene glycol Nanoparticles Cisplatin
