摘要
目的:了解黄芪注射液对加速度(+G z)导致大鼠肺损伤的作用,为临床通过药物手段提高加速度耐力提供理论依据。方法:雄性W istar大鼠20只,随机分为10G z对照组和+10G z黄芪组、15G z对照组和+15G z黄芪组(n=5)。对照组腹腔注射生理盐水10 ml/kg,用药组腹腔注射黄芪注射液10 g/kg。注射后1h分别经历+10Gz和+15Gz暴露,采用梯形加速度曲线,+10G z暴露90 s,+15G z暴露30 s,并间隔30 m in重复3次。于暴露后4h取大鼠腹腔静脉血、肺泡灌洗液(BALF)及肺组织,测定血清和BALF中TNF-α和IL-1β、肺组织TNF-α和IL-1βmRNA表达水平。结果:反复高+Gz作用后,黄芪组较对照组大鼠血清和BALF中TNF-α和IL-1β含量及其在肺组织中mRNA基因表达明显减少,+10Gz作用时差异非常明显。结论:黄芪注射液能减少反复高+Gz导致的大鼠炎症细胞因子TNF-α和IL-1β水平及其在肺组织中的基因表达,表明黄芪对反复高+G z引起的肺部损伤可能具有一定的保护作用。
Objective：To study the effects for Astragalus （AST） on its protection of the rats from lung injure induced by the sustained high ＋ Gz exposure, so as to offer the theory basis of exploiting the Chinese traditional medicine to improve the pilots＇ acceleration endurance. Methods： Twenty male healthy Wistar rats were randomly divided into 4 groups （ n = 5） ： control group for ＋ 10Gz and ＋ 15Gz, AST intervention group for ＋ 10Gz and ＋ 15Gz. For control group 0, 9% saline were injected into celiac cavity by 10ml/kg, and the AST were injected in AST groups by 10 g/kg. After one hour, the rats were exposed to ＋ 10Gz and ＋ 15Gz. Profile of the centrifuge ＋ Gz exposures was trapezoidal, ＋ 10Gz lasted for 90s, ＋ 15Gz group for 30s. The ＋ Gz repeated for 3 times with 30 min interval. The blood samples, Bronchoalveolar Lavage Fluid（BALF） and lung tissue homogenate were collected 4h after the last centrifuge run. The concentrations of TNF-α and IL-1β in the serum and BALF and their mRNA expression levels in rat lungs were measure＆ Results： For the AST convention group, the concentrations of TNF-α and IL-1β in the serum and BALF and their mRNA expression levels in the rat lungs were significantly lower than those of controis. Conclusion：ASY injection can alleviate inflammation of rats＇lung after high ＋ Gz exposure, which demonstrated AST could protect the rats from lung injure induced by the sustained high ＋ Gz exposure.
出处
《军医进修学院学报》
CAS
北大核心
2007年第1期68-70,共3页
Academic Journal of Pla Postgraduate Medical School
关键词
黄芪
加速度
细胞因子类
基因表达
Astragalus （AST）
acceleration
cytokines
gene expression
