摘要
目的观察神经妥乐平和恩在适对动物脑缺血和腹腔注射醋酸所致疼痛扭体反应的改善作用。方法建立大鼠大脑中动脉阻断再灌注模型,其中4组分别按10和20 U.kg-1的剂量给予神经妥乐平和恩在适,模型组和假手术组不给予任何药物,将各组大鼠的神经症状、脑梗死面积和脑水肿改善情况作为评价药物的指标;将小鼠腹腔注射醋酸致疼痛扭体反应,从小鼠对化学刺激所引起的扭体反应次数来评价药物的镇痛作用。结果神经妥乐平(10和20 U.kg-1)可改善神经症状、缩小梗死面积并减轻脑水肿;恩在适20 U.kg-1剂量组脑含水量和梗死面积减少,10 U.kg-1剂量组梗死面积无缩小。无论大小剂量组均无改善神经症状作用。神经妥乐平20 U.kg-1和40 U.kg-1剂量组均能抑制腹腔注射醋酸后引起的扭体反应,与模型组比较差异有显著性。恩在适在同样剂量下无镇痛作用。结论神经妥乐平有改善脑缺血-再灌注的作用,而较高剂量的恩在适才有抗脑缺血的作用。神经妥乐平能抑制由醋酸引起的疼痛反应,恩在适在同样剂量下无镇痛作用。
Objective To compare the effects of neurotropin and enzaishi in combating brain ischemia in rats and relieving pain caused by intraperitonial injection of acetic acid in mice. Methods （ 1 ） Male Wistar rats were randomly divided into 6 equal goups ： ① model group, ②and ③neurotropin treatment groups, ④and⑤ enzazsht treatment groups, ⑥sham operation group. A medel of focal cerebral ischemia-reperfusion injury was set up in each of the animals in groups ① to ⑤. Rats of group ⑥ underwent a sham operation each. Rats of group ② and ③ were given each an Ⅳ injection of 10 and 20 U·kg^-1 of neurotropin, respectively. Rats of groups ④ and ⑤were given each an Ⅳ injection of 10 and 20 U·kg^-1 of enzaishi, respectively. Rats of groups ① and ⑥ were given no treatment. The behavioral scorings, areas of brain infarction and brain water content in rats of the different groups were used as indexes for the assessment of the effects of neurotropin and enzaishi. （2） 175 mice of the Kunming strain were randomly divided into 7 equal groups： ① model, ②low-, ③medium-④high-dose neurotropin, ⑤ low-,⑥medium-,⑦high-dose enzaishi. Mice of group ① were given each an intraperitoneal （IP） injection of an equivalent amount of 0.9% sodium chloride solution. Mice of groups ②③and ④ were given each an IP injection of 10,20 and 40 U·kg^-1 of neurotropin, respectively. Mice of groups ⑤⑥ and ⑦ were given each an IP injection of 10,20 and 40 U·kg^-1 of enzaishi, respectively. 0.5 hafter the injection, mice of all 7 groups were given each an IP injection of 0.2 mL of 0.6% acetic acid. The frequencies of writhing reflexes exhibited by the animals within 15 rain after the injection of acetic acid were registered and used to assess the analgesic effects of the drugs. Results （ 1 ） Neurotropin in doses of 10 and 20 U·kg^-1 was shown to ameliorate the nervous function, reduce cerebral infarction area and mitigate brain edema in the rats. enzaishi in a dose of 20 U·kg^-1 could decrease the brain water content and reduce cerebral infarction area. However, it could not reduce the cerebral infarction area at a dose of 10 U·kg^-1. The nervous function was not improved by enzashi in both low and high doses. （2） Neurotropin in doses of 20 and 40 U·kg^-1 was shown to strikingly inhibit the writhing reflex in mice after an IP injection of acetic acid, the difference between the frequencies of writhing reflexes in mice of the model group and treated group being significant（ P 〈 0.05 ）. enzaishi in the same doses, however, had no effect on the writhing reflex. Conclusion Neurotropin in low and medium doses was shown to mitigate the brain injury caused by ischemia-reperfusion in rats. enzaishi, however, could combat brain ischemia only at the medium dose. Neurotropin in medium and high doses was shown to exert a striking analgesic effect in mice given IP injection of acetic acid, while enzaishi in the same doses had no such effect.
出处
《医药导报》
CAS
2007年第2期149-152,共4页
Herald of Medicine
关键词
神经妥乐平
恩在适
脑缺血-再灌注
镇痛
Neurotropin
Enzaishi
Ischemia-reperfusion injury, fecal cerebral
Analgesic effect
