摘要
目的研究铅对大鼠钙的吸收和骨骼生长的影响。方法分别以纯净水和1.0g/L醋酸铅水饲喂Wistar大鼠,其中醋酸铅饲喂大鼠又分别以正常饲料、高钙饲料和低钙饲料饲养,对应分组为对照组、单铅染毒组、高钙铅染毒组和低钙铅染毒组。观察大鼠生长发育,测定大鼠血清中骨钙素和甲状旁腺激素的含量,检测骨骼中铅和钙的含量,石蜡切片HE染色比较大鼠股骨显微结构变化。另饲养10只Wistar大鼠,以45CaCl2示踪研究铅对钙吸收的影响。结果铅和低钙处理明显抑制大鼠生长发育,使大鼠股骨的长度和直径减小,且低钙加剧了铅对骨骼的抑制作用,而高钙则拮抗铅对骨骼的抑制作用。示踪结果显示铅可显著抑制大鼠体内钙的吸收。结论铅可能通过抑制大鼠体内钙的吸收而使骨骼生长受限,补充适量钙有助于减轻铅对骨骼生长的抑制。
Objective To study the effect of lead on the calcium absorption and bone development in weanling rats. Methods Totally 80 weanling Wistar rats were equally divided into normal control （given with pure water and standard feed, including 1.15% calcium）, lead group （given 1.0 g/L lead acetate water and standard feed）, lead ＋ low calcium group （ 1.0 g/L lead acetate water and feed including 0.69% calcium）, and lead ＋ high calcium group （ 1.0 g/L lead acetate water and feed including 1.72 % calcium）. The development of rats was observed. Serum contents of osteocalcin and parathormone, and bone levels of lead and calcium in the femur were determined. The femur was examined with histological method. Another 5 Wistar rats received gastric irrigation of 10% lead acetate for 5 d, 5 more rats served as control, and then their absorption of calcium was detected with ^45CaCl2. Results Lead and low calcium inhibited the development of rat remarkably, with the content of osteocalcin, the length and diameter of the femur decreased. High calcium antagonized these effects of lead. The absorption of calcium in rats was repressed by lead. Conclusion Bone depression by lead may be due to that lead inhibits the absorption of calcium in rats, and the supplement of calcium is helpful to minimize the repression.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第5期402-405,共4页
Journal of Third Military Medical University
基金
中国营养学会营养科研基金(2003)~~
