摘要
将猫的心、脑、舌用盐酸-胃蛋白酶溶液消化处理后接种小白鼠,经连续传代分离弓形虫虫株,用特异PCR方法对所分离的虫株进行了鉴定。分别从24只猫的样品中分离出8株弓形虫虫株,用弓形虫种特异的PCR方法对8个虫株进行鉴定,均得到弓形虫的特异条带;测序证明所扩增出的DNA片段确为弓形虫的核糖体DNA第一内转录间隔区(ITS-1)部分序列。研究结果表明,将动物组织用盐酸-胃蛋白酶溶液消化处理后接种小白鼠是一种分离弓形虫虫株较为理想的方法,特异PCR方法能准确、快速地鉴定弓形虫虫株。
The objective of the present study was to isolate Toxoplasma gondii strains from cats and then to identify these strains by specific PCR assay. The T. gondii strains were isolated by inoculation in Kunming mice with cat tissues（the heart,brain and tongue） which were digested by acid pepsin solution, followed by specific amplification of partial ITS 1 rDNA using species-specific primers. In total, eight T. gondii strains were isolated from 24 cats,and the validity of the strains was confirmed by sequencing of the specific PCR products. These results demonstrated that the method of inoculation in the mice with the tissues that was digested by acid pepsin solution is an effective way to isolate T. gondii strain from animals, and the specific PCR assay is an accurate method for the rapid identification of T. gondii.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2007年第2期160-162,共3页
Chinese Veterinary Science
基金
国家杰出青年科学基金项目(30225033)
广东省自然科学基金团队项目(05200638)
关键词
弓形虫
猫
虫株分离
特异PCR
鉴定
Toxoplasma gondii
cat
isolation
specific PCR assay
identification
