摘要
目的：进一步探讨糖皮质激素防治肾间质纤维化的机理。方法：在成功分离培养人肾间质成纤维细胞基础上，通过流式细胞仪分析、ｄＵＴＰ标记ＤＮＡ断裂点显色及电镜技术等方法，观察甲基强的松龙对肾间质成纤维细胞编程性死亡的影响。结果：甲基强的松龙处理组编程性死亡的细胞百分数明显高于对照组，且呈时间依赖性。结论：甲基强的松龙可促进人肾间质成纤维细胞的凋亡。
BJECTIVESRecently,ithasbenshownthatabnormalgrowthandapoptosisoffibroblastsplayapivotalroleinrenalinterstitialfibrogenesis.Withtheestablishmentofhumanrenalinterstitialfibrob-lastscultures,weobservedtheefectsofglucocorticoidsontheprogrammedceldeathofthesehumanfibroblasts.METHODOLOGYHumanrenalinterstitialfibroblastsderivedfromkidneyswithinterstitialfibrosiswereculturedrandomlywithorwithout10-3Mmethylprednisolone.Programmedceldeathwasdetect-edwithflowcytometricDNAanalysis,dUTPlabelingDNAbreakingpointsandelectronicmicroscopicobservation.RESULTSMethylprednisolonesignificantlypromotedtheapoptosisofrenalinterstitialfibroblastsinvitroinatime-dependentmannerascomparedtothecontrol.Apoptoticcelsdetectedbyflowcytome-trywere1.8%and25.8%oftotalcelsat12hours;2.3%and68.9%at24hoursforthecontrolgroupandmethylprednisolonetreatedgrouprespectively.CONCLUSIONSItwasindicatedthatinducingapoptosisoffibroblastsmaybeanimportantmecha-nismformethylprednisolonetopreventrenalinterstitialfibrosis.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
1996年第6期5-8,共4页
Chinese Journal of Nephrology,Dialysis & Transplantation
