摘要
目的建立LC-MS联用测定人血浆中盐酸舍曲林血药浓度的方法。方法采用Waters AlltimaTMC18柱(100mm×2．1 mm,3．0μm),柱温30℃;流动相:0．1％甲酸溶液-乙腈=40:60(V/V);流速0．2 mL·min-1;进样量2止;气动辅助电喷雾离子化(ESI),正离子检测,SRM,盐酸舍曲林:[M+H]离子m/z 306．1,碎片子离子m/z 275．1,碰撞能量18 V;内标:盐酸帕罗西汀[M+H]离子m/z 330．1,碎片子离子m/z 192．1,碰撞能量27 V。结果该法专属性好,对盐酸舍曲林的最低检测限为0．673μg·L-1,血浆样品检测的线性、精密度、回收率等指标均符合生物样品分析标准,在应用中取得良好的效果。结论本法可用于血浆中盐酸舍曲林的测定。
AIM To develop a LC-MS method for determination of sertraline hydrochloride in human plasma. METHODS The method was performed on Waters Atlantis TMC18 column（ 100mm × 2.1 mm, 3.0 μm）at 30℃, 0.1% formic acid-acetonitrile（40： 60, V/V）was used as mobile phase with the flow rate of 0.2 mL·min^-1, the injection volume was 2 μL. Electrospray ionization（ESI） source was applied and operated in positive ion mode. Selected reaction monitoring （SRM）mode was used to quantify sertraline hydrochloride, The parent ion of sertraline hydrochloride and internal standard paroxetine[ M ＋ HI m/z were 306.1 and 330.1, respectively, while the product ion for sertraline hydrochloride and internal standard were 275.1 and 192.1, respectively. RESULTS This method was specific, the limit of detection was 0.673 μg·L^-1. CONCLUSION The method can be applied to determine sertraline hvdrochloride in human olasma.
出处
《中国临床药学杂志》
CAS
2007年第1期34-36,共3页
Chinese Journal of Clinical Pharmacy
