摘要
目的:探讨葡萄籽中的原花青素(GSP)对同型半胱氨酸(HCY)诱导的血管平滑肌细胞(VSMC)增殖、迁移的抑制作用及其机制。方法:采用细胞计数及[3H]-TdR检测细胞增殖,用DCFH-DA、Western blotting法检测GSP对VSMC增殖、迁移的抑制作用及分析其相关的分子信号转导通路。结果:HCY浓度依赖性地诱导VSMC的增殖与迁移,1 mmol/L HCY使VSMC的增殖与迁移分别比对照组升高3.9倍及4.1倍(P<0.01);DCFH-DA结果显示,1 mmol/L HCY使VSMC的活性氧(ROS)水平比对照组升高4倍(P<0.01)。而在不同浓度的GSP(5-20 g/L)处理组,HCY诱导VSMC的增殖、迁移以及活性氧水平都被显著抑制(P<0.01)。在GSP 20 g/L处理组,VSMC的增殖、迁移以及活性氧水平都接近空白对照组。与HCY组相比,GSP还显著降低HCY诱导VSMCMCP-1、IL-6及TNF-α的表达水平(P<0.01)。GSP还阻断HCY诱导的NF-κB的激活及显著降低NF-κB的活性(P<0.01)。结论:GSP通过抑制NF-κB的激活而抑制HCY诱导的血管平滑肌细胞增殖、迁移及炎症反应。
AIM： To investigate the effects of grasp seed procyanidlns （GSP） on homocysteine -induced proliferation and migration in vascular smooth muscle cells （VSMC） and related molecular mechanisms. METHODS： Cell count and [^3H ] - TdB assay were used for detecting cell proliferation and DNA synthesis, ELISA assay was used for detec- ting inflammatory response, DCFH -DA assay for examining the levels of reactive oxygen species （BOS）, Western blotting for detecting protein expression. RESULTS： Homocysteine （0. 1 - 1 mmol/L） increased VSMC proliferation and migration, and the levels of BOS were in a dose - dependent manner. The results of Western blotting showed that homocysteine significantly increased the expression of MCP - 1, IL - 6 and TNF - α. However, Compared with control group, in GSP （5 -20 g/L） group, the increased VSMC proliferation, migration and the production of BOS and the expression of MCP - 1, IL - 6 and TNF - α mediated by homooysteine were markedly suppressed. EMSA showed that in GSP treatment group, the NF-κB activation was also almost completely inhibited. CONCLUSION： GSP inhibits homocysteine -induced VSMC proliferation, migration and inflammatory response through interfering with BOS dependent on NF - κB signal pathway.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第2期325-330,共6页
Chinese Journal of Pathophysiology
关键词
原花青素
高半胱氨酸
平滑肌细胞
NF-ΚB
活性氧
Grasp seed procyanidins
Homocysteine
Smooth muscle cells
NF - kappa B
Beactive oxygen species
