摘要
目的考察3种大孔树脂和聚酰胺对地锦草总黄酮、金银花叶总黄酮和杭白菊总黄酮的吸附分离性能,以探讨大孔树脂等在精制地锦草、金银花叶和杭白菊有效部位的应用。方法以总黄酮为考察指标,筛选适用的大孔树脂等型号,评价大孔树脂的吸附与解吸工艺。用分光光度法测定总黄酮含量。结果地锦草、金银花叶和杭白菊总黄酮精制的适用树脂分别为D101、DA201、DM301;DA201;D101、DA201、DM301。地锦草总黄酮对DA201和对D101、金银花叶总黄酮对DA201、杭白菊总黄酮对DA201和对D101的上样浓度分别为0.49-1.47和0.42-1.31、1.03-2.07、0.50-1.00和0.71-1.99mg/m l,动态吸附阶段的泄漏点分别为8和10、2、2和1BV,乙醇洗脱液中总黄酮含量较高的乙醇浓度分别为20%、30%、40%和20%、30%、40%;10%、20%、30%;30%、40%、50%和20%、30%、40%。温度对DA201和D101吸附地锦草总黄酮影响不大,而对DA201或D101吸附金银花叶和杭白菊总黄酮有一定影响。结论3种大孔树脂和聚酰胺对中药总黄酮有效部位的提取精制有较大差别。
Objective In order to study the application of macroporous resins and so on to the purified active components of Euphorbia humifusa.Leaves of Flos Lonicerae. Chrysanthemum morifolium, adsorption and separation properties for 3 types of macroporous resins and polyamide were investigated. Methods The total flavone was used as the evaluating criteria,we selected suitable macroporous resins and studied optimum technological parameters of the adsorption and elution. Spectrophotometry was used for the determination of total flavone. Results The suitable macroporous resins which were used to the purified active components of traditional Chinese medicine were D101 and DA201 and DM301 for Euphorbia humifusa.DA201 for Leaves of Flos Lonicerae.Dt0, and DA20, and DM301 for Chrysanthemum morifoliurn,The concentration of the sample of Euphorbia humifusa for DA201 and D101 were 0.49 -1.47 and 0.42 - 1.31 mg/ml. The concentration of the sample of Leaves of Flos Lonicerae for DA204 was 1.03 - 2.07 mg/ml. The concentration of the sample of Chrysanthemum morifolium for Dja01 and D101 was 0.50 - 1.00 and 0.71 - 1.99 mg. ml. In the adsorption course ,appeared leaking were 8 and 10.2.2 and 1 BV respectively. In the elution course,when the alcohol concentrations were 20% .30% .40% and 20% .30% .40% ;10% .20% .30% ;30% . 40% .50% and 20% .30% .40% ; respectively,the total flavone content in the elution solutions was higher. The influence of temperature to DA201 and D101 adsorpting total flavone for Euphorbia humifusa was not great. But the influence of temperature to DA201 and D101 adsorpting total flavone for Leaves of Flos Lonicerae and Chrysanthemum morifolium were certain degree. Conclusions It is obviously different to refine the total flavone active components of traditional Chinese medicine, while using 3 types of macroporous resins and polyamide.
出处
《医学研究杂志》
2007年第1期56-59,共4页
Journal of Medical Research
基金
浙江省自然科学基金资助项目(Y205366)
关键词
地锦草
金银花叶
杭白菊
总黄酮
大孔树脂
Euphorbia humifusa
Leaves of Flos Lonicerae
Chrysanthemum morifolium
Total flavone
Macroporous resins
