摘要
目的构建缺氧诱导模型和观察缺氧对人结肠癌SW480细胞系生长和缺氧诱导因子-1α(hypoxiainduciblefactor-1alpha,HIF-1α),血红素氧合酶-1(hemeoxygenase-1,HO-1)表达的影响。方法利用氯化钴(cobaltchloride,CoCl2)构建缺氧诱导模型,以MTT试验观察不同程度缺氧条件对SW480细胞生长曲线的影响;利用RT-PCR方法测定氯化钴缺氧诱导与SW480细胞HIF-1α和HO-1基因mRNA表达的时效和量效关系。结果适度缺氧(CoCl2浓度<200μmol/L)可刺激肿瘤细胞增殖,过度缺氧(CoCl2浓度>250μmol/L)则抑制其生长;HIF-1α、HO-1基因mRNA表达与氯化钴缺氧呈明显的量效关系和时效关系;HIF-1α与HO-1基因mRNA的表达在缺氧诱导量效关系(相关系数r=0.786,P<0.05)和时效关系(相关系数r=0.863,P<0.05)中均存在显著的正相关性。结论缺氧可以刺激SW480细胞增殖;HIF-1α和HO-1基因的表达与肿瘤缺氧程度密切相关;HO-1的适量表达对缺氧细胞具有细胞保护作用,其过度表达可能导致细胞损伤和凋亡。
Objective： To create the hypoxia model and observe the reflectance of SW480 cell proliferation and HIF - 1α, HO - 1 gene mRNA expressions by hypoxia induced. Methods： The hypoxia model was set up by the usage of cobalt chloride （ CoCl2 ） and MTT test was used to observe the hypoxia influence of SW480 cell growth curve. RT - PCR was used to detect the HIF - 1α and HO - 1 gene mRNA expressions of SW480 cell which hypoxia induced by CoCl2 with different concentrations and times. Results： Hypoxia can stimulate the SW480 cell proliferation in a certain cobalt chloride concentration （ 〈200μmol/L） and inhibit cell growth with cobalt chloride concentration （ 〉 250μmol/L）. The HIF - lot and HO - 1 gene mRNA expressions had significant correlations with the CoCl2 concentration and hypoxia induced time. The HIF - lot had significant positive correlations with the HO - 1 genes mRNA expressions in the dose （ r = 0. 786, P 〈 0. 05 ） and time （ r = 0. 863, P 〈 0.05 ） dependence by hypoxia. Conelusion： Hypoxia can stimulate the SW480 cells proliferation. The expressions of HIF - lot and HO - 1 genes correlate with the degree of hypoxia. The HO - 1 gene moderate expression might protect tumor cells, but it＇s over expression would induce the tumor cell injury and apoptosis.
出处
《现代肿瘤医学》
CAS
2007年第2期161-164,共4页
Journal of Modern Oncology
