摘要
目的探讨肝细胞癌、肝硬化及正常肝组织中差异表达的基因并对其进行生物学信息分析。方法用Trizol一步法提取肝癌组织、肝硬化组织及正常肝组织的总RNA。并纯化mRNA,反转录合成荧光分子(Cy3/cy5)标记的cDNA探针,与含有4096个基因的芯片杂交;用Gene Pix Pro3.0图像分析软件分析肝硬化组织与正常肝组织,肝癌组织与正常肝组织的基因表达情况。结果与肝癌关系密切的基因有141条,许多与细胞周期和信号转导方面有关,如MCM7、YWHAH等。其中23条与在肝硬化组织中的表达趋势相同。与正常肝组织比较,有2条基因在癌组织中下调而在肝硬化组织中上调,均属于金属硫蛋白基因家族成员。结论在肝癌的发生、发展中,抑癌基因的失活、代谢相关酶类基因活性的异常及促使细胞进入增殖周期的相关基因的活化等因素发挥了重要的作用。系统的研究这些基因将有望发现新的早期诊断指标,为建立个体化治疗方案和预后评估系统提供帮助。
Objective To compare gene expression profiles among hepatocellular carcinomas（ HCC）, cirrhotic liver tissue and normal liver and to analyze their biological information. Methods Total RNA was isolated from hepatocellular carcinomas, cirrhotic liver tissue and normal liver respectively, purified into mRNA by oligotex, and reverse-transcribed to synthetize two kinds of fluorescences （Cy3-dUTP, CyS-dUTP） labeled cDNA probes. The targets were mixed together and hybridized with the genes on the genechip containing 4096 genes. The following comparisons were performed： hepatocellular carcinomas versus normal liver, cirrhotic liver versus normal liver. Genes that were differentially expressed between these groups were identified based on signal-to-noise ratios by using GenePix Pre3.0 software. Results 141 genes were found with close relation to HCCs, some of these genes were related to cell cycle and signal transduction, such as MCM7, YWHAH, among these, there are 23 genes whose expression tendency consistent with that of cirrhotic liver＇ s. When compared with normal liver, there were 2 genes belong to metallothionein family downregulated in HCCs but upregulated in cirrhotic liver. Conclusion The inactivation of anticancer genes, the abnormality of metabolism related genes and the activation of cell proliferation cycle related genes may have a vital role in HCC. Research into these genes systematically may help to explore new markers of early diagnosisand to guide treatment proposal and to evaluate prognosis better.
出处
《基础医学与临床》
CSCD
北大核心
2007年第1期57-62,共6页
Basic and Clinical Medicine
关键词
肝细胞癌
肝硬化
基因芯片
hepatocellular carcinoma
cirrhosis
genechip
