摘要
目的探讨检测肾脏病患者尿液中单核细胞趋化蛋白1（MCP-1）、壁层上皮细胞标志物β-catenin和角蛋白（CK）19分子的方法，并分析这些分子水平与肾小球新月体病变的关系。方法应用酶联免疫吸附试验（ELISA）方法，对正常人和肾脏病患者肾活检当日和部分患者肾活检后1个月晨尿中的MCP-1、β-catenin和CK19分子水平进行检测。将其结果与患者尿蛋白定量、。肾功能、肾组织中细胞性新月体指数和纤维性新月体指数进行相关分析。比较甲基泼尼松龙冲击治疗前后上述指标的变化。结果肾脏病患者尿MCP-1、β-catenin和CK19水平均较正常对照组显著升高（P〈0．01）。细胞新月体组患者尿MCP-1、β-catenin水平较无新月体组显著升高[（MCP-1水平Mr范围）247．54（22．17—2335．18）ng／mmol Cr比113．55（1．75～1057．77）ng／mmol Cr；尿β-catenin水平M（范围）219．40（0．93～3827．50）ng／mmol Cr比82．30（4．03。1632．58）ng／mmolCrP均〈0．01]。上述3种分子水平和细胞新月体指数呈正相关（r=0．75、0．21、0．63。P均〈0．01）。甲基泼尼松龙冲击治疗后，尿MCP-1、CK19水平较治疗前显著降低（M分别减少45％、57％，P〈0．01）。结论应用ELISA方法可以检测肾脏病患者尿MCP-1、β-catenin和CK19的水平，且尿液中上述分子水平与。肾组织细胞性新月体程度呈正相关。
Objective To detect the urinary levels of monocyte chemoattractant protein 1 （MCP-1）, β-catenin and cytokeratin 19 （CK19） in patients with various renal diseases and to analyze the relationship among these molecular levels in urine and the clinical and pathological features of patients, especially the formation of crescent. Methods Urine specimens of 20 healthy subjects and 124 patients with various renal diseases were collected at the day of renal biopsy and one month after renal biopsy respectively. Urinary levels of MCP-1, β-catenin and CK19 were examined by enzyme linked immunosorbent assay （ELISA）. Urinary creatinine, 24-hour urinary protein excretion and serum creatinine were detected at the same time. The indexes of cellular crescent and fibrous crescent were calculated according to the number and the size of crescents respectively. Results All the patients had significantly higher levels of urinary MCP-1, β-catenin and CK19 compared with healthy subjects （P 〈 0.01）.Levels of urinary MCP-1 and β-catenin were significantly higher in patients with cellular crescent than those in patients without crescent [median 247.54, range 22.17-2335.18 ng/mmol Cr vs median 113.55, range 1.75-1057.77 ng/ mmol Cr; median 219.40, range 0.93-3827.50 ng/mmol Cr vs median 82.30,range 4.03-1632.58 ng/mmol Cr, P〈0.01]. Urinary levels of MCP-1, β-catenin and CK19 were positively correlated to the indexes of cellular crescent （ r= 0.75,0.21,0.63, P〈 0.01）. Conclusions Urinary levels of MCP-1, CK19 and β-catenin can be detected By ELISA in healthy subjects and patients with various renal diseases. The levels of urinary MCP-1, β-catenin and CK19 are correlated to the indexes of cellular crescent in the renal biopsy specimens.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2007年第1期18-22,共5页
Chinese Journal of Nephrology
基金
首都医学发展科研基金资助项目（2005-3015）
感谢北京大学第一医院儿科和中日友好医院肾内科、儿科医护人员在标本收集工作中给予的大力支持
感谢北京大学第一医院皮肤科、检验科的老师在实验中提供的帮助
感谢北京大学第一医院统计室姚晨、李雪迎老师给予的指导
