摘要
依据羟乙基哌嗪乙烷磺酸(HEPES)能诱导增强肾综合征出血热病毒感染的Vero-E6细胞产生细胞融合病变,并可被特异性抗体所中和抑制的特性,建立了微量细胞病变中和试验方法。应用该方法对11株肾综合征出血热病毒分离株进行分型。结果7株可鉴定为HTN型,4株为SEO型。另对7个地区82份出血热病人血清进行分型。结果,西安、四川、沈阳、湖南、浙江的血清鉴定为HTN型;山西、山东、福建的血清定为SEO型。除三例外,其它每份血清抗体滴度两型间均相差4倍以上。各地区血清抗体水平的几何平均滴度(GMT)两型间相差10倍以上。毒株分型和各地区感染病毒型试验结果与作者用空斑减少中和试验(PRNT)分型的结果一致,但本方法具有较PRNT法试验时间短、容易掌握和经济等优点。
Based on our previous finding that HEPES could enhance Hantavirus infected Vero-E6 cells to cell fusion CPE and be inhibited by specific Hantavirus immune serum, a micro-neutralization test (HEPES-MCPENT) method was set up in this study. Then this method was applied for typing of Hantavirus isolates and Hemorrhagic fever with renal syndrome (HFRS) patient's sera. Eleven isolates from different regions & hosts were rested, among them 7 could be identified as HTN type, 4 as SEO type. Seventy-five serum samples from seven provinces were tested, those from Xian, Sichuan, Hunan and Shenyang could be identified as HTN type virus infection and those from Shanxi, Shandong and Fujian were SEO type virus infection. The titer differances between both types were more than four folds in most serum samples. The results of isolate typing and regional Hantavirus type infection in this study are consistent with our previous testings by PRNT method. However this method may be easier to handle, time saving and with less cost than the plaque reduction neatralization (PRNT) method.
出处
《中华实验和临床病毒学杂志》
CSCD
1996年第4期358-360,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
肾综合征出血热
病毒
HEPES
分型
Hantavirus HEPES Neutralization Typing Hemorrhagic fever with renal sydrome
