摘要
目的探讨垂体瘤中凝血酶敏感蛋白-1（TSP-1）、CD36、转化生长因子β1（TGF—β1）的表达及其与肿瘤侵袭性的关系。方法取52例垂体瘤（侵袭性组24例，非侵袭性组28例）手术标本，采用RT-PCR检测TSP-1、CD36、TGF-β1的mRNA表达，Western blot方法检测TSP-1、TGF-β1蛋白表达，并分析其表达与垂体瘤侵袭性等因素之间的关系。结果与非侵袭组相比，侵袭组TSP-1 mRNA及蛋白表达较弱，TGF-β1 mRNA及蛋白表达则强于非侵袭性组（P〈0．05），两组间CD36 mRNA表达差异无显著性意义（P〉0．05）。肿瘤的大小组间、各内分泌功能分型组间TSP-1、TGF-β1、CD36差异均无显著性意义（P〉0．05）。结论TSP-1、TGF—β1 mRNA及蛋白表达与垂体瘤侵袭性密切相关，提示其可能在垂体瘤发生发展中起重要作用，并能影响垂体瘤的侵袭性。
Objective To explore the expressions ofthrombospondin-1 （TSP-1）, transforming growth factor β1 （TGF-β1）, CD36 in pituitary adenomas and investigate the relationships between the expressions and the invasiveness of adenomas. Methods The mRNA expressions of TSP-1, TGF-β1, CD36 in 52 surgical specimens （24 invasive cases; 28 noinvasive cases） of pituitary adenomas was measured using RT-PCR and Western blot method was used to detect the protein levels of TSP-1 and TGF-β1. The correlations between the expressions and invasiveness of adenomas were analyzed. Results In invasive group as compared with the noinvasive group, the expression of TSP-1 （mRNA： t=4.389, P=000; protein： t=4.207, P=0001） was lower, and the expression of TGF-β1 （mRNA： r= 3.356, P=004; protein： t=5.59, P〈0.0001） was stronger, and the mRNA expression of CD36 （t=2.279, P= 0.063） was not significantly different. No difference in the expressions of TSP-1, TGF-β1 and CD36 was seen between different groups of various tumor sizes and endocrine functional types. Conclusion TSP-1 and TGF-β1 maybe play an important role in the development of pituitary adenomas and regulate its invasiveness.
出处
《中华神经医学杂志》
CAS
CSCD
2007年第1期18-21,共4页
Chinese Journal of Neuromedicine
