摘要
目的通过检测异基因组织工程骨移植修复猪胫骨缺损术后T淋巴细胞亚群CD4+和CD8+细胞的变化,探索异基因组织工程骨的免疫原性。方法骨髓间充质干细胞(MSCs)体外培养,扩增,诱导成骨后与磷酸三钙(TCP)复合为组织工程骨,分自体和同种异体组,植入构建好的猪胫骨中段2.0cm的缺损处,对照组为纯TCP组,流式检测术前,术后3、7、14、28、56d外周血T淋巴细胞亚群变化。结果小型猪外周血T淋巴细胞亚群分别为CD4+(9.37±1.65)%,CD8+(38.43±1.62)%,CD4+CD8+(7.23±1.24)%;术后组内不同时间点CD4+和CD8+细胞测定值无显著变化,CD4+CD8+双阳性细胞在3d和7d增加明显(P<0.05);组间各时间点CD4+、CD8+和CD4+CD8+细胞无显著变化。结论术后T细胞亚群检测结果显示无明显的免疫排斥,推论异基因组织工程骨免疫原性低。
Objective To detect the dynamic changes of the postoperative CD4^＋ and CD8^＋ lymphocyte subpopulation in peripheral blood, and to understand the immune state after allogeneic tissue engineering bone （TEB） transplantation for repairing swine bone defect and estimate the immunogenicity of TEB. Methods Mesenchymal stem cells （MSCs） isolated from flank bone marrow were cultured and amplified in vitro, then loaded on tricalcium phosphate （TCP） and combined into TEB after osteoinduction. The TEB were implanted into the defect （20 mm in length） created in the mid-portion tibia of allogeneic and autogeneic group, while TCP implanted in that of control group. CD4 ^＋ and CD8^＋ in all minipigs were tested before operation and 3, 7, 14, 28 and 56 days postop by flow cytometry （FCM）. Results The percent of CD4^＋ , CD8^＋ , and CD4^＋ CD8^＋ dull＋ lymphocyte subsets in minipig peripheral blood were 9.37 ± 1.65, 38.43 ± 1.62, and 7.23 ± 1.24, respectively. The population of CD4 ^＋ and CD8 ^＋ lymphocytes at postoperative different times had no significant change, but the CD4 ^＋ CD8 ^＋ dull ^＋ lymphocyte in each group increased obviously at 3 and 7 days postop （ P 〈 0.05）. The dynamics ratio of CD4 ^＋ , CD8 ^＋ , and CD4 ^＋ CD8 ^＋ dull ^＋ lymphocyte subsets had no obvious difference between groups. Conclusion The change of peripheral blood T lymphocyte subsets suggest the rejection is significant low, which indicate the allogeneic TEB has very low immunogenicity.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2007年第1期66-69,共4页
Immunological Journal
基金
国家高技术研究发展计划(863)重大专项(2003AA205020)
重庆市科技攻关项目(2003-13-6)资助
关键词
异基因
组织工程骨
骨缺损
T淋巴细胞亚群
Allogene
Tissue engineering bone
Bone defect
Subsets of T lymphocyte
