摘要
目的 探讨骨髓基质干细胞 (MSCs)与生物衍生骨复合修复山羊胫骨的长段骨 -骨膜缺损 ,以及修复负重骨缺损的可行性。 方法 将 18只 12月龄健康杂种青山羊 (雌雄不限 ) ,制备成双侧胫骨中段 2 0 mm骨 -骨膜缺损模型 ,常规钢板螺钉固定 ;MSCs与生物衍生骨于体外复合培养 ;对同一只山羊将复合物植入右侧胫骨缺损处作为实验组 ,以单纯材料植入左侧胫骨缺损处作为对照组 ,空白组不植入任何材料 ;在 8、12、16和 2 4周各时间点分别行标本的大体观察、X线片观察和骨密度测试 ,比较其修复骨缺损的能力。 结果 大体观察、X线片和骨密度测试显示 :实验组 8周骨缺损部分修复 ,12、16周骨缺损完全修复 ,8、12和 16周其骨密度较对照组高 ,差异有统计学意义 (P<0 .0 5 ) ;2 4周实验组与对照组骨密度差异无统计学意义 ;空白组骨缺损未修复。 结论 组织工程骨早期修复骨缺损能力较强 ,且较单纯材料成骨量大、迅速 ,能够对负重骨缺损进行有效的修复。
Objective To investigate the ability to repair goat tibia defect with marrow stromal stem cells (MSCs) and bio-derived bone, and the feasibility of the compounds as bone substitute material. Methods MSCs were cultured with the bio-derived bone in vitro, and the 20 mm tibia defect of goat was made and fixed with plate. Eighteen goats were divided into experimental group, control group and blank group. The defects were not filled with anything in blank group, with tissue engineering bone in experimental group and bio-derived bone in control group. The repair capability was assessed by physical, X-ray and bone mineral density examinations 8,12,16, and 24 weeks after operation. Results In experimental group, the defects were partially repaired 8 weeks, and completely repaired 12 and 16 weeks; there was significant difference in bone density between experimental group and control group (P<0.05) 8,12 and 16 weeks, but no significant difference 24 weeks. The defects of blank group were not repaired 24 weeks. Conclusion The tissue engineering bone can efficiently repair bone defect, and its repair capability is better than that of bio-derived bone alone both in quantity and quality of bone formation.
出处
《中国修复重建外科杂志》
CAS
CSCD
2004年第2期83-87,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家高技术研究发展计划 (863)资助项目(2 0 0 2 AA2 0 50 1 1 )
(2 0 0 1 AA2 1 60 1 1 )
国家自然科学基金资助项目(39830 1 0 0 )
卫生部重点资助项目 (2 0 0 1 3546)~~
