摘要
目的探讨甲胎蛋白异质体(AFP-L3)对原发性肝癌(primary hepatocellular carcinoma,PHC)早期诊断的意义。方法根据小扁豆凝集素对甲胎蛋白(AFP)的亲和力不同,采用亲和免疫电泳印迹法检测52例原发性肝癌和57例良性肝病患者的AFP-L3的血清含量;以AFP-L3>15%作为PHC阳性的诊断标准。结果PHC患者的AFP-L3阳性率明显高于良性肝病组,差别有统计学意义(P<0.01);PHC患者的AFP-L3阳性率与AFP水平无正相关。AFP≤400μg/L和>400μg/L的PHC病例,AFP-L3阳性率分别是76.9%和84.6%,AFP-L3含量与血清AFP浓度无关;AFP含量与癌肿直径大小呈正相关(r=0.453,P<0.01)。在癌肿直径>2 cm的PHC病例,AFP-L3阳性率与癌肿直径无相关性(r=0.012,P>0.05)。结论AFP-L3对于低水平AFP的PHC,尤其是小肝癌的诊断具有重要的临床意义。
Objective To study the clinical significance of lectin-reactive alpha-fetoprotein （AFP-L3） in early detection of primary hepatocellular carcinoma（PHC）. Methods According to the different affinity of lentil lectin with alpha-fetoprotein （AFP）, sera from 52 patients with primary PHC and 57 patients with benign liver disease were measured by affinity immunoelectrophoresis blotting to evaluate serum levels of AFP-L3. Positive criterium is AFP-L3 〉15%. Results The positive rate of AFP-L3 in the patients with PHC was significantly higher than that in the patients with benign liver disease（ P 〈0.01）. In the cases of AFP≤400μg/L and AFP〉400μg/L, the positive rate of AFP-L3 was 76.9 % and 84. 6%, respectively. The level of AFP-L3 had no correlation with the level of AFP. The level of AFP had positive correlation with the tumor（ r = 0. 453, P 〈0.01）. In the cases of tumors that measured more than 2 cm in diameter, the positive rate of AFP-L3 had no correlation with the the tumor（ r = 0.012, P 〉0.05）. Conclusion Measurement of AFP-L3 is useful for early detection of PHC in the patients with low level AFP,especially in those with small PHC.
出处
《临床荟萃》
CAS
北大核心
2007年第2期84-86,共3页
Clinical Focus
基金
佛山市卫生局资助项目(No:2004224)
关键词
肝肿瘤
甲胎蛋白类
兵豆属植物
植物蛋白类
诊断
鉴别
liver neoplasms
alpha-fetoproteins
lens plant
plant proteins
diagnosis,differential
