摘要
本研究利用沙门氏杆菌属特异的invA基因序列,设计一对特异性的引物,通过PCR反应来检测多种样品中的沙门氏杆菌;反应的特异性和敏感性以及反应的最适条件等试验的结果显示:此PCR方法检测沙门氏菌属的特异性为100%;样品中活菌的检测限为1.43CFU/10g;反应的最佳退火温度是65℃;最适模板浓度范围在104～108copies/ml。本方法与传统的沙门氏杆菌检测方法以及最近报道的相关方法比较,具有简单、快速、特异性好和敏感性高、经济等特点,并可满足大批样品沙门氏杆菌筛选检测的要求。
In this study, a pair of specificity primers designed basis on the Salmonella＇s inv A genome sequence, then ,the tests of the specificity and sensitivity of Sabnonella and the optimal conditions of PCR （optimal anneal temperature, optimal concentration of templates, etc.） had been discussed. The results showed that the specificity of this method to detect Salmonella is 100% and the limit of detection is 1.43CFU/10g/sample, the best anneal temperature of the PCR test is 65℃, the best template concentration is between 10^4～10^8copies/ml. The result of the experiments demonstrated that the PCR assay is a rapid, simple, economic and more specific, sensitive to traditional culture for the detection of 8abnonella in the food samples, and available for screening of Salmonella in a large number of food samples.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第12期607-611,共5页
Food Science
基金
国家自然科学基金重点项目(31031040)
