摘要
目的：建立反高效液相色谱法测定花锚中落干酸和龙胆苦苷的含量。方法：采用kromasil—C18（250mm×4．6mm，5μm）色谱柱，以流动相甲醇和水（含0．04％磷酸）的比例在0～15min内由23：77至30：70线性梯度洗脱，流速1ml／min，检测波长238nm，柱温35℃。结果：两种成分均达到基线分离，落干酸和龙胆苦苷的线性范围分别为0．198～5．544μg（r=0．9999），0．0262～0．7336μg（r=0．9999）；回收率为96．12％（RSD=1．73％）、97．67％（RSD=1．91％）。结论：方法测定快速，结果准确、可靠。
Objective： To establish a quantitative method of simultaneously determination of loganic acid and gentiopicroside by RP-HPLC. Method ：The sample wee separated on the column of kromasil-C18 （250mm × 4,6mm, 5μm）which eluted with methanol and water （0.04 % phosphoric acid）, The ratio of methanol and water increased from 23：77 to 30：70 in 15 min with detected wavelength at 238nm, flow rate at 1 ml/min, column temperature at 350C. Results：Two compounds were baseisolated. The linear ranges of loganic acid, gentiopicroside were 0.198- 5. 544 μg（ r= 0.9999）, 0.0262= 0,7336μg（ r = 0.9999） ; The average recoveries were 96.12 % （RSD = 1.73 % ） ,97.67 % （RSD = 1.91% ）. Conclusions： The method is rapid and precise.
出处
《微量元素与健康研究》
CAS
2007年第1期42-43,共2页
Studies of Trace Elements and Health
